Daza, RafaelCruces González, JuliaArroyo Hernández, MaríaMarí-Buyé, NúriaFuente del Rey, Mónica de laPlaza, Gustavo R.Elices Calafat, ManuelPérez Rigueiro, JoséGuinea, Gustavo V.2023-06-182023-06-182015-03-191478-3967, ESSN: 1478-397510.1088/1478-3975/12/2/026005https://hdl.handle.net/20.500.14352/23315The mechanical behavior of living murine T-lymphocytes was assessed by atomic force microscopy (AFM). A robust experimental procedure was developed to overcome some features of lymphocytes, in particular their spherical shape and non-adherent character. The procedure included the immobilization of the lymphocytes on amine-functionalized substrates, the use of hydrodynamic effects on the deflection of the AFM cantilever to monitor the approaching, and the use of the jumping mode for obtaining the images. Indentation curves were analyzed according to Hertz's model for contact mechanics. The calculated values of the elastic modulus are consistent both when considering the results obtained from a single lymphocyte and when comparing the curves recorded from cells of different specimens.engTopographical and mechanical characterization of living eukaryotic cells on opaque substrates: development of a general procedure and its application to the study of non-adherent lymphocytesjournal articlehttp://iopscience.iop.org/article/10.1088/1478-3975/12/2/026005/metarestricted access577.27577.2Atomic force microscopyCellMechanicsInmunologíaBiología celular (Biología)2412 Inmunología2407 Biología Celular