San Mauro Martín, IsmaelLópez Oliva, SaraGaricano Vilar, ElenaSánchez Niño, Guerthy MelissaPenadés, Bruno FTerrén Lora, AnaSanz Rojo, SaraCollado Yurrita, Luis Rodolfo2025-12-102025-12-102024-04-20San Mauro Martín I, López Oliva S, Garicano Vilar E, Sánchez Niño GM, Penadés BF, Terrén Lora A, Sanz Rojo S, Collado Yurrita L. Effects of Gluten on Gut Microbiota in Patients with Gastrointestinal Disorders, Migraine, and Dermatitis. Nutrients. 2024 Apr 20;16(8):1228. doi: 10.3390/nu16081228. PMID: 38674918; PMCID: PMC11053402.10.3390/NU16081228https://hdl.handle.net/20.500.14352/128704As gluten may trigger gastrointestinal disorders (GIDs), its presence or absence in the diet can change the diversity and proportion of gut microbiota. The effects of gluten after six weeks of a double-blind, placebo-controlled intervention with a gluten-free diet (GFD) were studied in participants with GIDs suffering from migraines and atopic dermatitis (n = 46). Clinical biomarkers, digestive symptoms, stool, the Migraine Disability Assessment questionnaire, and zonulin levels were analyzed. Next-generation sequencing was used to amplify the 16S rRNA gene of bacteria and the internal transcribed spacer (ITS) regions of fungi. The GFD increased Chao1 fungal diversity after the intervention, while the fungal composition showed no changes. Bacterial diversity and composition remained stable, but a positive association between bacterial and fungal Chao1 diversity and a negative association between Dothideomycetes and Akkermansia were observed. GIDs decreased in both groups and migraines improved in the placebo group. Our findings may aid the development of GID treatment strategies.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3390/nu16081228https://www.mdpi.com/2072-6643/16/8/1228open access616.34:579.61MIDASBacteriaFungiGastrointestinal disordersGlutenMicrobiotaZonulinGastroenterología y hepatologíaMicrobiología médica3205.03 Gastroenterología3201.03 Microbiología Clínica