Posada-Ayala, MariaÁlvarez Llamas, GloriaMaroto, Aroa S.Maes, XavierMuñoz-Garcia, EstherVillalba Díaz, María TeresaRodríguez García, RosalíaPerez-Gordo, MarinaVivanco Martínez, FernandoPastor Vargas, CarlosCuesta-Herranz, Javier2024-07-242024-07-242015Posada-Ayala M, Alvarez-Llamas G, Maroto AS, Maes X, Muñoz-Garcia E, Villalba M, Rodríguez R, Perez-Gordo M, Vivanco F, Pastor-Vargas C, Cuesta-Herranz J. Novel liquid chromatography-mass spectrometry method for sensitive determination of the mustard allergen Sin a 1 in food. Food Chem. 2015 Sep 15;183:58-63. doi: 10.1016/j.foodchem.2015.02.139. Epub 2015 Mar 12. Erratum in: Food Chem. 2016 Sep 1;206:291. doi: 10.1016/j.foodchem.2016.03.090. PMID: 25863610.0308-814610.1016/j.foodchem.2015.02.139https://hdl.handle.net/20.500.14352/107107Mustard is a condiment added to a variety of foodstuffs and a frequent cause of food allergy. A new strategy for the detection of mustard allergen in food products is presented. The methodology is based on liquid chromatography analysis coupled to mass spectrometry. Mustard allergen Sin a 1 was purified from yellow mustard seeds. Sin a 1 was detected with a total of five peptides showing a linear response (lowest LOD was 5ng). Sin a 1 was detected in mustard sauces and salty biscuit (19±3mg/kg) where mustard content is not specified. Sin a 1, used as an internal standard, allowed quantification of this mustard allergen in foods. A novel LC/MS/MS SRM-based method has been developed to detect and quantify the presence of mustard. This method could help to detect mustard allergen Sin a 1 in processed foods and protect mustard-allergic consumers.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.foodchem.2015.02.139https://www.sciencedirect.com/science/article/pii/S0308814615003428open access577.1577.2Hidden allergenLC–MSMustard allergyQuantificationSRMAllergensSin a 1Bioquímica (Química)Biología molecular (Química)2403 Bioquímica2302.21 Biología Molecular