Serafín González-Carrato, VerónicaArévalo Pérez, BeatrizMartínez-García, GonzaloAznar-Poveda, JuanLopez-Pastor, José AntonioBeltrán-Sánchez, J. FranciscoGarcia-Sanchez Antonio, JavierGarcia-Haro, JuanCampuzano Ruiz, SusanaYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel2024-01-252024-01-252019Serafín V, Arévalo B, Martínez-García G, Aznar-Poveda J, Lopez-Pastor J.A, Beltrán-Sánchez J.F, Garcia-Sanchez A.J, Garcia-Haro J, Campuzano S, Yáñez-Sedeño P, Pingarrón J.M. Enhanced determination of fertility hormones in saliva at disposable immunosensing platforms using a custom designed field-portable dual potentiostat. Sens. Actuators, B. 2019 Agosto 7; 299: 1269340925-400510.1016/j.snb.2019.126934https://hdl.handle.net/20.500.14352/95389This work describes a new electroanalytical device for the simultaneous and reliable determination of two fertility relevant hormones (luteinizing hormone, LH, and progesterone, P4) in saliva. The device is constructed using a custom designed and field-portable potentiostat where dual disposable immunosensing platform are connected. The immunosensors are based on sandwich-type and competitive immunoassays implemented onto magnetic microbeads (MBs) functionalized with Neutravidin and Protein G for the determination of LH and P4, respectively. Amperometric detection performed at −0.20 V vs the Ag pseudo-reference electrode using the H2O2/hydroquinone (HQ) system was employed as the transduction technique after placing the MBs with immunocomplexes for each target hormone on the appropriate working electrode of screen-printed dual carbon electrodes (SPdCEs). The method exhibits high sensitivity and selectivity for the target hormones providing detection limits of 1.7 pg mL−1 and 0.10 mIU mL−1 for P4 and LH, respectively, with a 1 h test time. The applicability of the method was confirmed by determining both hormones in saliva samples from different volunteers providing results comparable to those obtained using amperometric transduction with a commercial potentiostat and with ELISA methodologies involving the same immunoreagents for each target hormone.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.snb.2019.126934https://produccioncientifica.ucm.es/investigadores/142687/colaboracion/investigador/1822507?lang=enrestricted access544.6Dual immunosensorFertility hormonesMagnetic beadsDual immunosensorSalivaField-portable potentiostatQuímica analítica (Química)2301 Química Analítica