Abellanas Perez, PedroCarballares, DiegoRocha Martín, JavierFernandez Lafuente, Roberto2025-06-122025-06-122023-08Abellanas-Perez P, Carballares D, Rocha-Martin J, Fernandez-Lafuente R. The nature of the buffer alters the effects of the chemical modification on the stability of immobilized lipases. Process Biochemistry 2023;133:20–7. https://doi.org/10.1016/j.procbio.2023.08.003.1359-511310.1016/j.procbio.2023.08.003https://hdl.handle.net/20.500.14352/121295The objective of this paper was to analyze whether an interaction between the effects of the buffer nature and chemical modification on enzyme stability exists. For this, the lipase B from Candida antarctica (CALB) and the lipase from Thermomyces lanuginosus (TLL) were immobilized on octyl agarose beads and modified with picryl sulfonic acid (TNBS) and ethylenediamine via the carbodiimide route. The obtained biocatalysts were them inactivated in different buffers (2-amino-2-(hydroxymethyl)propane-1,3-diol (Tris)-HCl, N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) (HEPES) or phosphate). A significant interaction was found between the chemical modification of the enzyme surfaces and their stabilities in these different buffers. While phosphate was the buffer where the lowest enzyme stabilities were found for both unmodified immobilized enzymes, the differences on enzyme stabilities become much smaller after some chemical modifications. In many instances, chemical modification improves enzyme stability when using a buffer and was negative when using the other one (e.g., TNBS modification of TLL was positive using Tris-HCl or negative using the other buffers, amination of CALB decreased its stability when inactivated in Tris-HCl or HEPES while it almost had no effect in phosphate). Thus, clear co-interactions of the effects oengAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1873-3298https://doi.org/10.1016/j.procbio.2023.08.003https://www.sciencedirect.com/science/article/pii/S1359511323002635open access577.15Immobilized lipase stabilitySolid phase chemical modification of lipasesEffect of the buffer nature on enzyme stabilityBioquímica (Biología)2403 Bioquímica2302.09 Enzimología