Kubánková, MarkétaSummers, Peter A.López Duarte, IsmaelKiryushko, DaryaKuimova, Marina K.2025-01-232025-01-232019-09-12Kubánková M, Summers PA, López-Duarte I, Kiryushko D, Kuimova MK. Microscopic viscosity of neuronal plasma membranes measured using fluorescent molecular rotors: effects of oxidative stress and neuroprotection. ACS Appl Mater Interfaces [Internet]. 9 de octubre de 2019 [citado 23 de enero de 2025];11(40):36307-15. Disponible en: https://pubs.acs.org/doi/10.1021/acsami.9b104261944-82441944-825210.1021/acsami.9b10426https://hdl.handle.net/20.500.14352/115748Molecular mobility in neuronal plasma membranes is a crucial factor in brain function. Microscopic viscosity is an important parameter that determines molecular mobility. This study presents the first direct measurement of the microviscosity of plasma membranes of live neurons. Microviscosity maps were obtained using fluorescence lifetime imaging of environment-sensing dyes termed “molecular rotors”. Neurons were investigated both in the basal state and following common neurodegenerative stimuli, excitotoxicity, or oxidative stress. Both types of neurotoxic challenges induced microviscosity decrease in cultured neurons, and oxidant-induced membrane fluidification was counteracted by the wide-spectrum neuroprotectant, the H3 peptide. These results provide new insights into molecular mobility in neuronal membranes, paramount for basic brain function, and suggest that preservation of membrane stability may be an important aspect of neuroprotection in brain insults and neurodegenerative disorders.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1021/acsami.9b10426open access615.31615:34neuronsplasma membranemolecular rotormicroviscosityfluorescence lifetime imagingoxidative stressexcitotoxicityneuroprotectionH3 peptideQuímica farmaceútica23 Química