Bañares, CeliaAntezana, Ana VictoriaChabni, AssamaeRamirez-Veliz, CarlosIntiquilla, ArturoJiménez-Aliaga, KarimZavaleta, Amparo I.Hernández-Ledesma, BlancaTorres, Carlos F.Gómez-Cortés, Pilar2026-03-192026-03-192026Bañares, C., Antezana, A. V., Chabni, A., Ramirez-Veliz, C., Intiquilla, A., Jiménez-Aliaga, K., Zavaleta, A. I., Hernández-Ledesma, B., Torres, C. F., & Gómez-Cortés, P. (2026). Revalorization of Lupinus mutabilis seed oil via clean expeller extraction and comprehensive chemical characterization. Journal of Food Composition and Analysis, 152, 109023. https://doi.org/10.1016/j.jfca.2026.1090230889-157510.1016/j.jfca.2026.109023https://hdl.handle.net/20.500.14352/134149Credit authorship contribution statement Celia Bañares: Writing – original draft, Methodology, Investigation, Formal analysis, Data curation. Ana Victoria Antezana: Writing – original draft, Methodology, Investigation, Formal analysis, Data curation. Assamae Chabni: Writing – original draft, Investigation, Formal analysis, Data curation. Carlos Ramirez-Veliz: Methodology, Investigation. Arturo Intiquilla: Writing – review & editing, Methodology, Conceptualization. Karim Jiménez-Aliaga: Methodology, Investigation. Amparo I. Zavaleta: Resources, Investigation. Blanca Hernández-Ledesma: Writing – review & editing, Supervision, Resources, Project administration, Funding acquisition, Conceptualization. Carlos F. Torres: Writing – review & editing, Supervision, Resources, Project administration, Methodology, Funding acquisition, Conceptualization. Pilar Gómez Cortés: Writing – review & editing, Supervision, Resources, Project administration, Funding acquisition, Conceptualization.This study aims to revalorize Lupinus mutabilis (L. mutabilis) seed oil through expeller extraction method and its extensive characterization. Oil recovery accounted for 44 % based on the total fat content of L. mutabilis seeds (25 %). The oil showed an initial peroxide value of 4.1 meq O2/kg, p-anisidine value of 1.0, and TOTOX value of 9.2, all within acceptable limits for vegetable edible oils. The fatty acid (FA) composition was predominantly unsaturated, with oleic acid (cis-9 C18:1, 44 g/100 g) and linoleic acid (cis-9, cis-12 C18:2, 34 g/100 g) as major FAs. For the first time, the positional distribution of FAs in the triacylglycerol backbone was determined, showing a balanced presence of oleic and linoleic acids in the sn-2 and sn-1,3 positions. Additionally, this study reports for the first time the quantification (11 g/100 g) and characterization of the phospholipid (PL) fraction. The main PL was phosphatidylcholine, followed by phosphatidylethanolamine and lysophosphatidylcholine. The total phenolic content was 99 mg gallic acid equivalents (GAE)/kg oil. The oil also contained 7120 mg/kg of sterols and 494 mg/kg of α-tocopherol. These results demonstrate the potential of expeller-extracted L. mutabilis seed oil for nutraceutical applications, as a natural source of health-promoting lipids and bioactive compoundsengAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1096-0481https://doi.org/10.1016/j.jfca.2026.109023open access664.3:613.2Lupin seedsOil expeller extractionOxidative statusChemical compositionPolar lipidsDietética y nutrición (Farmacia)3206 Ciencias de la Nutrición