Romero Sánchez, IvánRamírez García, LorenaGracia Lor, EmmaMadrid Albarrán, María Yolanda2023-06-222023-06-222022-07-110308-814610.1016/j.foodchem.2022.133611https://hdl.handle.net/20.500.14352/71776CRUE-CSIC (Acuerdos Transformativos 2022)Rice is frequently contaminated with aflatoxins, that are highly toxic fungal substances and strongly involved on hepatic cancer. In this work, different extraction and clean-up methods were evaluated for the simultaneous extraction and clean-up of aflatoxins B1, B2, G1 and G2 from rice. Favourable results were obtained by using methanol – water (80:20, v/v) extraction followed by immunoaffinity columns for clean-up, with recoveries of 86–92%, standard deviations between 5 and 11%, LOD ranged between 0.09 and 0.32 µg/kg, and LOQ between 0.31 and 1.06 µg/kg. Method validation and sample analysis were performed by using HPLC-MS/MS. Nine rice samples from different origin, varieties and specific characteristics, acquired in Spanish supermarkets were analysed. In two basmati samples from the same batch aflatoxin B1 was detected at (1.62 ± 0.08) µg/kg and (0.77 ± 0.03) µg/kg, both lower than the levels established by European Regulation for aflatoxin B1 in cereals.engAtribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/journal articlehttps://doi.org/10.1016/j.foodchem.2022.133611open accessAflatoxinsCerealsRiceImmunoaffinity columnsHPLC-MS/MSBioanalytical ChemistryQuímica analítica (Química)2301 Química Analítica