Lifante Cañavate, JoséShen, YingliZabala Gutiérrez, IreneRubia Rodríguez, IreneOrtega, DanielFernández Monsalve, NuriaMelle Hernández, SoniaGranado García, MiriamRubio Retama, Benito JorgeJaque García, DanielXimendes, Erving Clayton2023-06-172023-06-172021-03-03Lifante Cañavete, J., Shen, Y., Zabala Gutiérrez, I. et al. «Reaching Deeper: Absolute In Vivo Thermal Reading of Liver by Combining Superbright Ag 2 S Nanothermometers and In Silico Simulations». Advanced Science, vol. 8, n.o 9, mayo de 2021, p. 2003838. DOI.org (Crossref), https://doi.org/10.1002/advs.202003838.2198-3844 (online)10.1002/advs.202003838https://hdl.handle.net/20.500.14352/7891Online Version of Record before inclusion in an issue. Art. 2003838. Version of Record online: 03 March 2021 Manuscript revised: 23 December 2020 Manuscript received: 08 October 2020Luminescent nano‐thermometry is a fast‐developing technique with great potential for in vivo sensing, diagnosis, and therapy. Unfortunately, it presents serious limitations. The luminescence generated by nanothermometers, from which thermal readout is obtained, is strongly distorted by the attenuation induced by tissues. Such distortions lead to low signal levels and entangle absolute and reliable thermal monitoring of internal organs. Overcoming both limitations requires the use of high‐brightness luminescent nanothermometers and adopting more complex approaches for temperature estimation. In this work, it is demonstrated how superbright Ag2S nanothermometers can provide in vivo, reliable, and absolute thermal reading of the liver during laser‐induced hyperthermia. For that, a new procedure is designed in which thermal readout is obtained from the combination of in vivo transient thermometry measurements and in silico simulations. The synergy between in vivo and in silico measurements has made it possible to assess relevant numbers such as the efficiency of hyperthermia processes, the total heat energy deposited in the liver, and the relative contribution of Ag2S nanoparticles to liver heating. This work provides a new way for absolute thermal sensing of internal organs with potential application not only to hyperthermia processes but also to advanced diagnosis and therapy.daniel.jaque@uam.esengAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.1002/advs.202003838https://onlinelibrary.wiley.com/doi/10.1002/advs.202003838open access535.37539.2:620.1543.226Deep tissueLiverLuminiscenceNanoparticlesNano-thermometryÓptica (Física)BiotecnologíaTécnicas de la imagen2209.19 Óptica Física3399 Otras Especialidades Tecnológicas