Huarte, NereaCarravilla, PabloCruz Rodríguez, AntonioLorizate, MaierNieto-Garay, Jon A.Kräusslich, Hans-GeorgPérez-Gil, JesúsRequejo Isidro, JoséNieva, José L.2023-06-182023-06-1820162045-2322, ESSN: 2045-232210.1038/srep34190https://hdl.handle.net/20.500.14352/23379The chemical composition of the human immunodeficiency virus type 1 (HIV-1) membrane is critical for fusion and entry into target cells, suggesting that preservation of a functional lipid bilayer organization may be required for efficient infection. HIV-1 acquires its envelope from the host cell plasma membrane at sites enriched in raft-type lipids. Furthermore, infectious particles display aminophospholipids on their surface, indicative of dissipation of the inter-leaflet lipid asymmetry metabolically generated at cellular membranes. By combining two-photon excited Laurdan fluorescence imaging and atomic force microscopy, we have obtained unprecedented insights into the phase state of membranes reconstituted from viral lipids (i.e., extracted from infectious HIV-1 particles), established the role played by the different specimens in the mixtures, and characterized the effects of membrane-active virucidal agents on membrane organization. In determining the molecular basis underlying lipid packing and lateral heterogeneity of the HIV-1 membrane, our results may help develop compounds with antiviral activity acting by perturbing the functional organization of the lipid envelope.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://www.nature.com/articles/srep34190open access577.112Chemical biologyFluorescence imagingMembrane structure and assemblyViral membrane fusionBioquímica (Biología)2302 Bioquímica