Wojtusik, MateuszRodríguez Martín, AlbertoRipoll Morales, VanessaSantos Mazorra, Victoria E.García, Jose L.García-Ochoa Soria, Félix2023-06-192023-06-1920158756-7938 (Print) 1520-6033 (Online)10.1016/j.btre.2014.12.010https://hdl.handle.net/20.500.14352/34001Production of 1,3-propanediol from glycerol using Klebsiella oxytoca NRRL-B199 has been studied. Medium composition has been optimized by means of a statistical design based on the Taguchi method. Strong influences of glycerol and phosphate concentrations have been detected on biomass and product yields. Other factors, such as magnesium concentration and K:Na ratio, have shown a small influence on both responses, biomass and product concentrations. An optimized medium composition has been proposed, leading to a final 1,3-propanediol concentration of 12.4 g/L with a selectivity of 72% with respect to glycerol consumed at shaken bottle-scale. Once the medium composition had been optimized, the scale-up from shaken bottles to STBR was conducted. Several experiments in a 2 L STBR have been conducted in order to determine the best operating conditions concerning temperature and agitation. Under the best operating conditions, i.e., a programmed variable stirring rate ranging from 50 to 100 rpm and a temperature of 37 �C, a final concentration of 13.5 g/L of 1,3-propanediol with a selectivity of 86% with respect to the glycerol consumed was obtained.engAtribución-NoComercial-SinDerivadas 3.0 Españajournal articlehttp://www.sciencedirect.com/science/article/pii/S2215017X14000666open access66.013-Propanediol productionKlebsiella oxytocaMedium compositionOperating conditionsScale-upIngeniería química3303 Ingeniería y Tecnología Químicas