Carrión Herrero, Francisco JavierHorcajo Iglesias, María Del PilarDomínguez Bernal, Gustavo RamónFuente López, Ricardo De LaRuiz Santa Quiteria Serrano De La Cruz, José AntonioOrden Gutiérrez, José Antonio2024-01-182024-01-182012Horcajo, P., Domínguez-Bernal, G., Carrión, J., De La Fuente, R., Ruiz-Santa-Quiteria, J. A., & Orden, J. A. (2013). Differences in virulence gene expression between atypical enteropathogenic Escherichia coli strains isolated from diarrheic and healthy ruminants. Canadian journal of veterinary research = Revue canadienne de recherche veterinaire, 77(2), 158–160https://hdl.handle.net/20.500.14352/93859Differences in the pathogenicity of atypical enteropathogenic Escherichia coli (EPEC) strains may be due, at least partially, to different expression patterns of some virulence genes. To investigate this hypothesis, the virulence gene expression patterns of 6 atypical EPEC strains isolated from healthy and diarrheic ruminants were compared using quantitative real-time reverse transcription polymerase chain reaction after growing the bacteria in culture medium alone or after binding it to HeLa epithelial cells. Some virulence genes in strains from diarrheic animals were upregulated relative to their expression in strains from healthy animals. When bacteria were cultured in the presence of HeLa cells, the ehxA and efa1/lifA genes, previously associated with the production of diarrhea, were expressed at higher levels in strains from diarrheic animals than in strains from healthy animals. Thus, the expression levels of some virulence genes may help determine which atypical EPEC strains cause diarrhea in ruminants.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article0830-9000https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3605933/24082409open access636.09AnimalsCattleCattle DiseasesDiarrheaEnteropathogenic Escherichia coliGene Expression RegulationBacterialGoat DiseasesGoatsRNA, BacterialSheepSheep DiseasesVirulence FactorsVeterinaria24 Ciencias de la Vida