Sánchez Beltrán, María Del CarmenHerráiz, AnaCiudad Cabañas, María JoséArias, MartaAlonso, RaquelDoblas, CarmenLlama Palacios, María ArantxazuCollado Yurrita, Luis Rodolfo2025-10-302025-10-302024-07-13Sánchez, M. C., Herráiz, A., Ciudad, M. J., Arias, M., Alonso, R., Doblas, C., Llama-Palacios, A., & Collado, L. (2024). Metabolomics and Biochemical Benefits of Multivitamin and Multimineral Supplementation in Healthy Individuals: A Pilot Study. Foods, 13(14), 2207. https://doi.org/10.3390/foods131422072304-815810.3390/foods13142207https://hdl.handle.net/20.500.14352/125519Scientific evidence regarding the effectiveness of vitamin and mineral supplements in healthy individuals remains scarce. In a randomized, double-blind study, 30 healthy individuals were assigned to receive a single daily dose of multivitamin and multimineral supplementation or a double daily dose for 30 days. Before and after the intake, an untargeted metabolomics assay for serum metabolites was conducted by hydrophilic interaction liquid chromatography-mass spectrometry, and clinical assessments of peripheral blood samples were performed. A paired t-test for metabolic analysis, adjusted using the false discovery rate (FDR) and p-value correction method (rate of change > 2 and FDR < 0.05), the Shapiro-Wilk test, Student's t-test, and the Mann-Whitney U test were applied depending on the variable, with a 5% significance level. An impact on oxidative stress was observed, with a significant reduction in homocysteine levels and an increment of pyridoxic acid (vitamin B6). The effect on energy metabolism was shown by a significant increase in diverse metabolites, such as linoleoylcarnitine. Serum iron and calcium levels were also impacted. Overall, we observed a nutritional balance compatible with a good state of health. In conclusion, beneficial effects on adult health were demonstrated in relation to oxidative stress, energy metabolism, and nutritional balance.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps:// doi.org/10.3390/foods13142207https://www.mdpi.com/2304-8158/13/14/2207open access612.3961nutritional supplementmultivitamin complexmultimineral complexnatural origin vitamins and mineralshomocysteineironenergy metabolismpyridoxic acidlinoleoylcarnitineCiencias Biomédicas32 Ciencias Médicas