Anadón Navarro, Arturo RamónGamboa, FelipeMartínez Caballero, María AranzazuCastellano Santos, Víctor JesúsMartínez Caballero, MartaAres Lombán, IrmaRamos Alonso, EvaSuárez, Frank H.Martínez Larrañaga, María Rosa2024-02-012024-02-012012Anadón A, Gamboa F, Martínez MA, Castellano V, Martínez M, Ares I, Ramos E, Suarez FH, Martínez-Larrañaga MR. Plasma disposition and tissue depletion of chlortetracycline in the food producing animals, chickens for fattening. Food Chem Toxicol. 2012 Aug;50(8):2714-21. doi: 10.1016/j.fct.2012.05.007. Epub 2012 May 14. PMID: 22595330.0278-691510.1016/j.fct.2012.05.007https://hdl.handle.net/20.500.14352/97581Chickens were used to investigate plasma disposition of chlortetracycline after single IV (15 mg/kg) and multiple oral administration (60 mg/kg, 5 days) and residue depletion of chlortetracycline after multiple oral doses (60 mg/kg, 5 days). Plasma and tissue samples were analyzed by HPLC. Mean elimination half-lives in plasma were 7.96 and 13.15 h after IV and multiple oral administration. Maximum plasma concentration was 4.33 lg/ml and the interval from oral administration until maximal concentration was 1.79 h. Oral bioavailability was 17.76%. After multiple oral dose, mean kidney, liver and muscle tissue concentrations of chlortetracycline + 4-epi-chlortetracycline of 835.3, 192.7, and 126.3 ug/kg, respectively, were measured 1 day after administration of the final dose of chlortetracycline. Chlortetracycline residues were detected in kidney and liver (205.4 and 81.7 ug/kg, respectively), but not in muscle, 3 days after the end of chlortetracycline treatment. The mean chlortetracycline + 4-epi-chlortetracycline concentrations were below LOQ at 3 and 5 days after cessation of medication in muscle and liver, respectively. A withdrawal time of 3 days was necessary to ensure that the chlortetracycline residues were less than the maximal residue limits (MRLs) established by the European Union (100, 300, and 600 ug/kg in muscle, liver, and kidney, respectively).engjournal articlehttps://doi.org/10.1016/j.fct.2012.05.007restricted access636.09ChlortetracyclinePlasma and tissue depletionWithdrawal timeChickens for fatteningVeterinaria3214 Toxicología