Heras, ClaraJiménez Holguín, JavierDoadrio Villarejo, Antonio LuisVallet Regí, María Dulce NombreSánchez Salcedo, SandraSalinas Sánchez, Antonio Jesús2023-06-162023-06-162020-07-24Heras, C., Jiménez Holguín, J., Doadrio Villarejo, A. L. et al. «Multifunctional Antibiotic- and Zinc-Containing Mesoporous Bioactive Glass Scaffolds to Fight Bone Infection». Acta Biomaterialia, vol. 114, septiembre de 2020, pp. 395-406. DOI.org (Crossref), https://doi.org/10.1016/j.actbio.2020.07.044.1742-706110.1016/j.actbio.2020.07.044https://hdl.handle.net/20.500.14352/6421RESEARCHER ID D-8709-2017 (Antonio Doadrio Villarejo) ORCID 0000-0001-8380-4781 (Antonio Doadrio Villarejo) RESEARCHER ID N-4501-2014 (Sandra Sánchez Salcedo) ORCID 0000-0002-1889-2057 (Sandra Sánchez Salcedo) RESEARCHER ID M-3378-2014 (María Vallet Regí) ORCID 0000-0002-6104-4889 (María Vallet Regí) RESEARCHER ID M-3316-2014 (Antonio Salinas Sánchez) ORCID 0000-0002-8408-3389 (Antonio Salinas Sánchez)Bone regeneration is a clinical challenge which requires multiple approaches. Sometimes, it also includes the development of osteogenic and antibacterial biomaterials to treat the emergence of possible infection processes arising from surgery. This study evaluates the antibacterial properties of gelatin-coated meso-macroporous scaffolds based on the bioactive glass 80%SiO2–15%CaO–5%P2O5 (mol-%) before (BL-GE) and after being doped with 4% of ZnO (4ZN-GE) and loaded with both saturated and the minimal inhibitory concentrations of one of the antibiotics: levofloxacin (LEVO), vancomycin (VANCO), rifampicin (RIFAM) or gentamicin (GENTA). After physical-chemical characterization of materials, release studies of inorganic ions and antibiotics from the scaffolds were carried out. Moreover, molecular modelling allowed determining the electrostatic potential density maps and the hydrogen bonds of antibiotics and the glass matrix. Antibacterial in vitro studies (in planktonic, inhibition halos and biofilm destruction) with S. aureus and E. coli as bacteria models showed a synergistic effect of zinc ions and antibiotics. The effect was especially noticeable in planktonic cultures of S. aureus with 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and in E. coli cultures with LEVO or GENTA. Moreover, S. aureus biofilms were completely destroyed by 4ZN-GE scaffolds loaded with VANCO, LEVO or RIFAM and the E. coli biofilm total destruction was accomplished with 4ZN-GE scaffolds loaded with GENTA or LEVO. This approach could be an important step in the fight against microbial resistance and provide needed options for bone infection treatment.engjournal articlehttps://doi.org/10.1016/j.actbio.2020.07.044https://www.ucm.es/valletregigroupopen access546615.46Mesoporous bioactive glassesZnOBone infectionStaphylococcus aureusEscherichia coliMaterialesQuímica inorgánica (Farmacia)3312 Tecnología de Materiales