Mínguez Mínguez, Fernando A.Lima José EnriqueGarcía Esteban, María TeresaPrieto José2025-10-272025-10-271997Mínguez Mínguez, J.E. Lima, M.T. García, J. Prieto; Effects of Antifungal Pretreatment on the Susceptibility of Candida albicans to Human Leukocytes. Chemotherapy 1 May 1997; 43 (5): 346–351. https://doi.org/10.1159/0002395880009-315710.1159/000239588https://hdl.handle.net/20.500.14352/125376The aim of this work was to measure the susceptibility of Candida albicans pretreated for 2 or 6 h with fluconazole (Flu), ketoconazole (Ktz), amphotericin B (AmB) and 5-fluorocyto-sine (5-Fc) to the fungicidal action of human polymorphic leukocytes. The influence of pretreatment was measured by comparing the delay (in hours) and reduction (logio) in growth of pretreated cultures in the presence of leukocytes and serum with that of non-pretreated control cultures. Six-hour pretreat-ments with Flu, Ktz, AmB and 5-Fc led to growth delays of 1, 3, 3 and 3 h, respectively. No significant differences were found when pretreatment lasted only 2 h. With respect to a reduction in growth, this was larger when the preincubation was 6 h, mainly for 5-Fc and AmB. 5-Fc was seen to be the most effective, followed by Ktz, AmB and finally Flu. It may be concluded that antifungal pretreatment renders this yeast more susceptible to the action of leukocytes. The degree of susceptibility achieved is dependent on the antifungal agent employed.engjournal article1421-9794https://doi.org/10.1159/0002395889309368https://karger.com/che/article-abstract/43/5/346/65524/Effects-of-Antifungal-Pretreatment-on-the?redirectedFrom=fulltextrestricted access612.017579.62615.282616.09Amphotericin BFluconazoleKetoconazole5-FluorocytosineSusceptibility to leukocytesInmunologíaFarmacología (Medicina)Microbiología médicaOncología2414 Microbiología2412 Inmunología3209 Farmacología3201.01 Oncología