Romero, HéctorSerrano, EsterHernández-Tamayo, RogelioCarrasco, BegoñaCárdenas Mastrascusa, Paula PatriciaAyora, SilviaGraumann, Peter L.Alonso, Juan C.2025-01-082025-01-082020Romero, Hector, et al. «Bacillus subtilis RarA Acts as a Positive RecA Accessory Protein». Frontiers in Microbiology, vol. 11, febrero de 2020, p. 92. https://doi.org/10.3389/fmicb.2020.00092.1664-302Xdoi: 10.3389/fmicb.2020.00092https://hdl.handle.net/20.500.14352/113204Ubiquitous RarA AAAC ATPases play crucial roles in the cellular response to blocked replication forks in pro- and eukaryotes. Here, we provide evidence that absence of RarA reduced the viability of recA, recO, and recF15 cells during unperturbed growth. The rarA gene was epistatic to recO and recF genes in response to H2O2- or MMS-induced DNA damage. Conversely, the inactivation of rarA partially suppressed the HR defect of mutants lacking end-resection (addAB, recJ, recQ, recS) or branch migration (ruvAB, recG, radA) activity. RarA contributes to RecA thread formation, that are thought to be the active forms of RecA during homology search. The absence of RarA reduced RecA accumulation, and the formation of visible RecA threads in vivo upon DNA damage. When rarA was combined with mutations in genuine RecA accessory genes, RecA accumulation was further reduced in rarA recU and rarA recX double mutant cells, and was blocked in rarA recF15 cells. These results suggest that RarA contributes to the assembly of RecA nucleoprotein filaments onto single-stranded DNA, and possibly antagonizes RecA filament disassembly.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.3389/fmicb.2020.00092open access577579Mgs1WRNIP1Replication stressRecA mediatorsRecA modulatorsBiología molecular (Biología)Bioquímica (Biología)Microbiología (Biología)2403 Bioquímica2415.01 Biología Molecular de Microorganismos2414 Microbiología