Heras Márquez, DiegoRobles Martín, Ana.Amigot Sánchez, RafaelFernández López, LauraGonzález Alfonso, José LRoda, SergiAlcolea Rodríguez, VíctorHeras Márquez, DiegoAlmendral, DiegoCoscolín, CristinaPlou, Francisco JPortela, RaquelBañares, Miguel ÁngelMartínez Del Pozo, ÁlvaroGarcía Linares, SaraFerrer, ManuelGuallar, Víctor2024-12-172024-12-172023-10-23Robles-Martín, A., Amigot-Sánchez, R., Fernandez-Lopez, L. et al. Sub-micro- and nano-sized polyethylene terephthalate deconstruction with engineered protein nanopores. Nat Catal 6, 1174–1185 (2023). https://doi.org/10.1038/s41929-023-01048-610.1038/s41929-023-01048-6https://hdl.handle.net/20.500.14352/112760The identification or design of biocatalysts to mitigate the accumulation of plastics, including sub-micro- and nano-sized polyethylene terephthalate (nPET), is becoming a global challenge. Here we computationally incorporated two hydrolytic active sites with geometries similar to that of Idionella sakaiensis PET hydrolase, to fragaceatoxin C (FraC), a membrane pore-forming protein. FraCm1/m2 could be assembled into octameric nanopores (7.0 nm high × 1.6–6.0 nm entry), which deconstructed (40 °C, pH 7.0) nPET from GoodFellow, commodities and plastic bottles. FraCm1 and FraCm2 degrade nPET by endo- and exo-type chain scission. While FraCm1 produces bis(2-hydroxyethyl) terephthalate as the main product, FraCm2 yields a high diversity of oligomers and terephthalic acid. Mechanistic and biochemical differences with benchmark PET hydrolases, along with pore and nPET dynamics, suggest that these pore-forming protein catalytic nanoreactors do not deconstruct macro-PET but are promising in nanotechnology for filtering, capturing and breaking down nPET, for example, in wastewater treatment plants.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1038/s41929-023-01048-6https://www.nature.com/articles/s41929-023-01048-6open access577.1CienciasBioquímica (Química)23 Química