Sánchez-Fortún Rodríguez, SebastiánMartín Fernández, MargaritaCosta Buitrago, Gonzalo AntonioNande Barbeitos, María Del MarGarrido, ElenaFajardo Martínez, Carmen MaríaMartín, Carmen2023-06-222023-06-222022-03-12Fajardo, C., Martín, C., Garrido, E., Sánchez-Fortún, S., Nande, M., Martín, M., & Costa, G. (2022). Copper and Chromium toxicity is mediated by oxidative stress in Caenorhabditis elegans: The use of nanoparticles as an immobilization strategy. Environmental toxicology and pharmacology, 92, 103846. https://doi.org/10.1016/j.etap.2022.1038461382-668910.1016/j.etap.2022.103846https://hdl.handle.net/20.500.14352/72536Environmental contamination by heavy metals (HMs) has impelled searching for stabilization strategies, where the use of zero-valent iron nanoparticles (nZVI) is considered a promising option. We have evaluated the combined effect of Cu(II)-Cr(VI) on two Caenorhabditis elegans strains (N2 and RB1072 sod-2 mutant) in aqueous solutions and in a standard soil, prior and after treatment with nZVI (5% w/w). The results showed that HMs aqueous solutions had an intense toxic effect on both strains. Production of reactive oxygen species and enhanced expression of the heat shock protein Hsp-16.2 was observed, indicating increased HM-mediated oxidative stress. Toxic effects of HM-polluted soil on worms were higher for sod-2 mutant than for N2 strain. However, nZVI treatment significantly diminished all these effects. Our findings highlighted C. elegans as a sensitive indicator for HMs pollution and its usefulness to assess the efficiency of the nanoremediation strategy to decrease the toxicity of Cu(II)-Cr(VI) polluted environments.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.etap.2022.103846https://www.sciencedirect.com/science/article/pii/S1382668922000394?via%3Dihubopen accessSoilWaterHeavy metalsCaenorhabditis elegansNZVIROSQuímicaAguaEcología (Biología)Medio ambiente naturalFarmaciaFarmacología (Farmacia)Toxicología (Farmacia)23 Química2303.31 Química del Agua2401.06 Ecología animal3209 Farmacología6113.05 Tratamiento de la Drogadicción