Garcimartín Álvarez, AlbaMerino Martín, José JoaquínSantos López, Jorge ArturoLópez-Oliva Muñoz, María ElviraGonzález Prieto, María Del PilarSánchez Muniz, Francisco JoséBenedí González, Juana María2025-01-272025-01-272015Garcimartín A, Merino JJ, Santos-López JA, López-Oliva ME, González MP, Sánchez-Muniz FJ, Benedí J. Silicon as neuroprotector or neurotoxic in the human neuroblastoma SH-SY5Y cell line. Chemosphere. 2015 Sep;135:217-24. doi: 10.1016/j.chemosphere.2015.04.060. Epub 2015 May 15. PMID: 25957141.10.1016/j.chemosphere.2015.04.060. Epub 2015 May 15.https://hdl.handle.net/20.500.14352/116411Silicon (Si) is a trace element that has been considered to be an environmental contaminant for many years, although different studies have recently reported it is an essential element for living cells. The present study tested the ability of different concentrations of Si G57™ to induce neuroprotection or neurotoxicity over 24 h in the SH-SY5Y human neuroblastoma cell line. Cell viability, cellular proliferation, LDH release, ROS, antioxidant capacity, TBARS, caspase-3, -8 and -9, DNA fragmentation, and TNF-α levels were evaluated. Low Si doses (50-250 ng mL(-1)) increased the cell viability and reduced caspase-3 and -8 activities and TNF-α level. The increase in cell viability was independent of any proliferative effect as there was no variation in cyclin E and PCNA levels. At higher concentrations, Si increased caspase-3, as well as TBARS, LDH, DNA fragmentation, and TNF-α releases. Altogether, these results suggest that Si could act either as a neuroprotector or a neurotoxic agent depending on the concentration tested. This study emphasizes the importance of developing new neuroprotective therapies based on low Si doses.engjournal article1879-1298https://doi.org/10.1016/j.chemosphere.2015.04.060https://pubmed.ncbi.nlm.nih.gov/25957141/restricted accessCell deathSH-SY5YCiencias24 Ciencias de la Vida