De Pablo Moreno, Juan AndrésLiras Martín, AntonioRevuelta Rueda, Luis2024-01-242024-01-242022-03-28De Pablo-Moreno, J. A., Liras, A., & Revuelta, L. (2022). Standardization of Coagulation Factor V Reference Intervals, Prothrombin Time, and Activated Partial Thromboplastin Time in Mice for Use in Factor V Deficiency Pathological Models. Frontiers in Veterinary Science, 9, 8462162297-176910.3389/fvets.2022.846216https://hdl.handle.net/20.500.14352/95130Author Contributions JADPM contributed to the design of the experiments and participated in the collection, analysis, and interpretation of the data. He also contributed to drafting the manuscript and preparing the figures and tables. AL and LR contributed to the conception and design of the experiments, participated in the data analysis, contributed the reagents, materials and analytical tools, collaborated in drafting the manuscript and preparing the figures and tables, and reviewed successive drafts of the paper. AL is the principal investigator of the project and obtained the funds required for the project. All authors have read and agreed to the published version of the manuscript.Factor V together with activated factor X forms the prothrombinase complex, which transforms prothrombin into thrombin. The Mus musculus species is characterized by very high levels of this factor and short clotting times, which hinders accurate measurements. For that reason, a detailed characterization of such parameters is indispensable. A method was designed as part of this study to provide an accurate determination and standardization of factor V levels, prothrombin time and activated partial thromboplastin time in Mus musculus. Those parameters were evaluated in a sample of 66 healthy animals using a semi-automated coagulometer and human diagnostic reagents in an attempt to determine the most appropriate time of day for the extractions. A mouse-based protocol was designed, capable of making corrections to the samples at dilutions of 1:100 for factor V and at of 1:3 for prothrombin time. The goal was to smoothen the calibration curves, which often present with steep slopes and narrow measurement ranges between one calibration point and another. It was found that the most stable period for blood sample extraction was that comprised between the first 6 h of light. No clinical differences were observed between the sexes and reference intervals were established for factor V (95.80% ± 18.14; 25.21 s ± 1.34), prothrombin time (104.31% ± 14.52; 16.85 s ± 1.32) and activated partial thromboplastin time (32.86 s ± 3.01). The results obtained are applicable to human or veterinary biomedical research, to transfusional medicine or to pathological models for diseases such as factor V deficiency.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://www.frontiersin.org/articles/10.3389/fvets.2022.846216/fullopen access636.09Factor VProthrombin timeActivated partial thromboplastin timeMouseMeasurement standardization, transfusionPathological modelsFactor V deficiencyVeterinaria3109 Ciencias Veterinarias