Hernáiz Gómez-Degano, María JosefaLevin, G.Gómez, S.Glodowsky, A.Cascone, O.Hernáiz Gómez-Degano, María Josefa2024-01-292024-01-292018-100141-813010.1016/j.ijbiomac.2018.05.177https://hdl.handle.net/20.500.14352/95781A rapid and efficient enzymatic procedure for the preparation of an immobilized beta-galactosidase has been described. In a first step, soybean peroxidase was used to catalyze the polymerization of a strategically activated phenol (N-Succinimidyl 3-(4-hydroxyphenyl)propionate, known as Bolton-Hunter reagent). The phenolic support was directly employed for immobilizing S-galactosidase from Bacillus circulans (ATCC 31382, beta-Gal-3), giving rise to a new biocatalyst subsequently applied in the synthesis of a beta-galatodisaccharide (Gal-beta(1-3)-GlcNAc and Gal-beta(1-3)-GalNAc). The reaction proceeded with high conversion rates and total regioselectivity. Reusability assays were performed with the same reaction conditions finding that the immobilized enzyme retains about 55% of its activity after eight batches. Finally and based on our results, the two-step enzymatic procedure presented here is a good and green alternative to the preparation of carbohydrates with biological activities.engjournal articlehttps://doi.org/10.1016/j.ijbiomac.2018.05.177open accessEnzymatic polymerizationSoybean peroxidaseEnzyme immobilizationβ-GalactosidaseTransglycosylationCiencias23 Química