Li, ShaoguangFerrer-Ruiz, AndrésDai, JunRamos-Soriano, JavierDu, XueweiZhu, ManZhang, WanxueWang, YuanyuanHerranz Astudillo, María ÁngelesJing, LeZhang, ZishuoLi, HuiXia, FanMartín León, Nazario2025-05-132025-05-132022-06-27Li, S. Ferrer-Ruiz, A. Dai, J. Ramos-Soriano, J. Du, X. Zhu, M. Zhang, W. Wang, Y. Herranz, M.i. Jing, L. Zhang, Z. Li, H. Xia, F. Martín, N. A pH-independent electrochemical aptamer-based biosensor supports quantitative, real-time measurement in vivo. Chem. Sci., 2022, 13, 8813-8820.10.1039/D2SC02021Ahttps://hdl.handle.net/20.500.14352/120027Electronic supplementary information (ESI) available. See https://doi.org/10.1039/d2sc02021aThe development of biosensors capable of achieving accurate and precise molecular measurements in the living body in pH-variable biological environments (e.g. subcellular organelles, biological fluids and organs) plays a significant role in personalized medicine. Because they recapitulate the conformation-linked signaling mechanisms, electrochemical aptamer-based (E-AB) sensors are good candidates to fill this role. However, this class of sensors suffers from a lack of a stable and pH-independent redox reporter to support their utility under pH-variable conditions. Here, in response, we demonstrate the efficiency of an electron donor p-extended tetrathiafulvalene (exTTF) as an excellent candidate (due to its good electrochemical stability and no proton participation in its redox reaction) of pH-independent redox reporters. Its use has allowed improvement of E-AB sensing performance in biological fluids under different pH conditions, achieving high-frequency, real-time molecular measurements in biological samples both in vitro and in the bladders of living rats.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://pubs.rsc.org/en/content/articlelanding/2022/sc/d2sc02021ahttps://produccioncientifica.ucm.es/documentos/62f80c329162cc4ea7f1e9c4open access547BiosensorAptamerPH-independentRedoxElectron donorTetrathiafulvaleneQuímica orgánica (Química)2306 Química Orgánica