Mulder, JanAguado Sánchez, TaniaKeimpema, ErikBarabás, KlaudiaBallester Rosado, CarlosNguyen, LaurentMonory, KrisztinaMarsicano, GiovanniDi Marzo, VincenzoHurd, YasminGuillemot, FrancoisMackie, KenLutz, BeatGuzmán Pastor, ManuelLu, Hui ChenGalve Roperh, IsmaelHarkany, Tibor2023-12-202023-12-202008Mulder, Jan, et al. «Endocannabinoid Signaling Controls Pyramidal Cell Specification and Long-Range Axon Patterning». Proceedings of the National Academy of Sciences, vol. 105, n.o 25, junio de 2008, pp. 8760-65. https://doi.org/10.1073/pnas.0803545105.0027-842410.1073/pnas.0803545105https://hdl.handle.net/20.500.14352/91636Endocannabinoids (eCBs) have recently been identified as axon guidance cues shaping the connectivity of local GABAergic interneurons in the developing cerebrum. However, eCB functions during pyramidal cell specification and establishment of long-range axonal connections are unknown. Here, we show that eCB signaling is operational in subcortical proliferative zones from embryonic day 12 in the mouse telencephalon and controls the proliferation of pyramidal cell progenitors and radial migration of immature pyramidal cells. When layer patterning is accomplished, developing pyramidal cells rely on eCB signaling to initiate the elongation and fasciculation of their long-range axons. Accordingly, CB1 cannabinoid receptor (CB1R) null and pyramidal cell-specific conditional mutant (CB1Rf/f,NEX-Cre) mice develop deficits in neuronal progenitor proliferation and axon fasciculation. Likewise, axonal pathfinding becomes impaired after in utero pharmacological blockade of CB1Rs. Overall, eCBs are fundamental developmental cues controlling pyramidal cell development during corticogenesis.engjournal article1091-6490https://doi.org/10.1073/pnas.0803545105restricted access577.1616.8ExcitationGlutamateLayer patterningNeocortexNeurogenesisBioquímica (Biología)Biología celular (Biología)Neurociencias (Biológicas)2403 Bioquímica2407 Biología Celular2490 Neurociencias