Carpena Torres, CarlosSchilling, TimHuete Toral, FernandoBahmani, HamedCarracedo Rodríguez, Juan Gonzalo2024-01-112024-01-112023-07-26Carpena Torres, C., Schilling, T., Huete Toral, F. et al. «Increased Ocular Dopamine Levels in Rabbits after Blue Light Stimulation of the Optic Nerve Head». Experimental Eye Research, vol. 234, septiembre de 2023, p. 109604. DOI.org (Crossref), https://doi.org/10.1016/j.exer.2023.109604.0014-483510.1016/j.exer.2023.109604https://hdl.handle.net/20.500.14352/92480The purpose was to quantify ocular dopamine in rabbits after stimulation of the optic nerve head with short-wavelength (blue) light to activate melanopsin expressed in the axons of intrinsically photosensitive retinal ganglion cells (ipRGCs). Dopamine levels in tears, aqueous humor, vitreous body, and retina (including choroid) were quantified after blue light stimulation of the optic nerve head of 15 rabbits with an optical fiber for 1 min, 10 min, or no stimulation (n = 5, each group). The left eye of all rabbits was operated on to introduce the optical fiber and stimulate the optic nerve, while the contralateral eye served as internal control. One minute of blue light stimulation significantly increased dopamine concentration in the vitreous body of the treated eyes compared to the contralateral ones (P = 0.015). Stimulation for 10 min significantly increased dopamine concentration in the vitreous body, as well as the aqueous humor (P < 0.05). Therefore, using an optical fiber approach to stimulate the optic nerve head with blue light significantly increased dopamine concentration in the aqueous humor and the vitreous body. This likely reflects an upregulation of retinal dopamine synthesis that could be attributed to ipRGC activation. However, the data provided in this study fell short of establishing a definitive link between dopamine release and ipRGC activation, mainly due to the absence of evidence supporting the expression of the melanopsin photopigment in the optic nerve.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal articlehttps://doi.org/10.1016/j.exer.2023.109604https://www.sciencedirect.com/science/article/pii/S0014483523002257#:~:text=In%20rabbits%2C%20the%20stimulation%20of,in%20the%20tears%20or%20retina.open access617.7Blue lightDopamineDopaminergic amacrine cellsIntrinsically photosensitive retinal ganglion cellsMelanopsinMyopiaÓptica y optometría2209.15 Optometría