Utrero Rico, AlbertoGonzález Cuadrado, CeciliaChivite Lacaba, MartaCabrera Marante, ÓscarLaguna Goya, RocíoAlmendro Vázquez, PatriciaDíaz Pedroche, CarmenRuiz Ruigómez, MaríaLalueza Blanco, AntonioFolgueira López, María DoloresVázquez, EnriqueQuintas, AnaBerges Buxeda, Marcos J.Martín Rodriguez, MoisésDopazo, AnaSerrano Hernández, AntonioAguado García, José MaríaPaz Artal, Estela Natividad2023-06-162023-06-162021-09-10Utrero Rico, A., González Cuadrado, C., Chivite Lacaba, M. et al. «Alterations in Circulating Monocytes Predict COVID-19 Severity and Include Chromatin Modifications Still Detectable Six Months after Recovery». Biomedicines, vol. 9, n.o 9, septiembre de 2021, p. 1253. DOI.org (Crossref), https://doi.org/10.3390/biomedicines9091253.2227-905910.3390/biomedicines9091253https://hdl.handle.net/20.500.14352/5048This study was supported by the Instituto de Salud Carlos III, Spanish Ministry of Science and Innovation (COVID-19 research call COV20/00181)—co-financed by the European Development Regional Fund “A way to achieve Europe” and by Consejería de Sanidad de la Comunidad de Madrid (CÍVICO study 2020/0082). R.L.G. and O.C.M. hold a research contract “Rio Hortega” (CM19/00120 and CM19/00092, respectively) from the Instituto de Salud Carlos III, Spanish Ministry of Science and Innovation. MCL holds a predoctoral fellowship (FPU19/06393) from the Spanish Ministry of Science and Innovation.An early analysis of circulating monocytes may be critical for predicting COVID-19 course and its sequelae. In 131 untreated, acute COVID-19 patients at emergency room arrival, monocytes showed decreased surface molecule expression, including low HLA-DR, in association with an inflammatory cytokine status and limited anti-SARS-CoV-2-specific T cell response. Most of these alterations had normalized in post-COVID-19 patients 6 months after discharge. Acute COVID-19 monocytes transcriptome showed upregulation of anti-inflammatory tissue repair genes such as BCL6, AREG and IL-10 and increased accessibility of chromatin. Some of these transcriptomic and epigenetic features still remained in post-COVID-19 monocytes. Importantly, a poorer expression of surface molecules and low IRF1 gene transcription in circulating monocytes at admission defined a COVID-19 patient group with impaired SARS-CoV-2-specific T cell response and increased risk of requiring intensive care or dying. An early analysis of monocytes may be useful for COVID-19 patient stratification and for designing innate immunity-focused therapies.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.3390/biomedicines9091253https://www.mdpi.com/2227-9059/9/9/1253open access616.9Circulating monocytesCOVID-19HLA-DRTranscriptomeChromatin accessibilityMedicinaEnfermedades infecciosas32 Ciencias Médicas3205.05 Enfermedades Infecciosas