Chowen, Julie A.Rábano, AlbertoVázquez Pérz, PatriciaRoncero Rincón, IsabelÁlvarez García, ElviraSanz Miguel, María Del CarmenBlázquez Fernández, EnriqueWiley2024-01-292024-01-292004-02-04Roncero I, Alvarez E, Chowen JA, et al. Expression of glucose transporter isoform GLUT-2 and glucokinase genes in human brain. J Neurochem. 2004;88(5):1203-1210. doi:10.1046/j.1471-4159.2003.02269.x0022-30421471-415910.1046/j.1471-4159.2003.02269.xhttps://hdl.handle.net/20.500.14352/96203The glucose transporter isoform-2 (GLUT-2) and glucokinase are considered to be components of a glucose sensor system controlling several key processes, and hence may modulate feeding behaviour. We have found GLUT-2 and glucokinase mRNAs in several brain regions, including the ventromedial and arcuate nuclei of the hypothalamus. GLUT-2, glucokinase and glucokinase regulatory protein mRNAs and proteins were present in these areas as determined by biochemical approaches. In addition, glucose-phosphorylating activity with a high apparent Km for glucose that displayed no product inhibition by glucose-6-phosphate was observed. Increased glycaemia after meals may be recognized by specific hypothalamic neurones due to the high Km of GLUT-2 and glucokinase. This enzyme is considered to be the true glucose sensor because it catalyses the rate-limiting step of glucose catabolism its activity being regulated by interaction with glucokinase regulatory protein, that functions as a metabolic sensorengAttribution 4.0 Internationaljournal articlehttps://onlinelibrary.wiley.com/doi/full/10.1046/j.1471-4159.2003.02269.x?sid=nlm%3Apubmedopen access612.017Ciencias Biomédicas24 Ciencias de la Vida