Zhu, LongfeiDluzewska, JuliaFernández Jiménez, NadiaRanjan, RajeevPelé, AlexandreDziegielewski, WojciechSzymanska-Lejman, MajaHus, KarolinaGórna, JuliaPradillo Orellana, MónicaZiolkowski, Piotr A.2024-11-272024-11-272024Longfei Zhu, Julia Dluzewska, Nadia Fernández-Jiménez, Rajeev Ranjan, Alexandre Pelé, Wojciech Dziegielewski, Maja Szymanska-Lejman, Karolina Hus, Julia Górna, Mónica Pradillo, Piotr A Ziolkowski, The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis, The Plant Cell, 2024;, koae292, https://doi.org/10.1093/plcell/koae2921040-465110.1093/plcell/koae292https://hdl.handle.net/20.500.14352/111140This work was supported by the National Science Center Poland grants 2016/22/E/NZ2/00455 and 2020/39/I/NZ2/02464 to P.A.Z. The computations were carried out in the Poznan Supercomputing and Networking Center (grants 312 and pl0074-02). W.D. and M.Sz.L. are the holders of Foundation for Polish Science stipends (START 2023).Meiotic crossover, i.e. the reciprocal exchange of chromosome fragments during meiosis, is a key driver of genetic diversity. Crossover is initiated by the formation of programmed DNA double-strand breaks (DSBs). While the role of ATAXIA-TELANGIECTASIA AND RAD3-RELATED (ATR) kinase in DNA damage signaling is well-known, its impact on crossover formation remains understudied. Here, using measurements of recombination at chromosomal intervals and genome-wide crossover mapping, we showed that ATR inactivation in Arabidopsis (Arabidopsis thaliana) leads to dramatic crossover redistribution, with an increase in crossover frequency in chromosome arms and a decrease in pericentromeres. These global changes in crossover placement were not caused by alterations in DSB numbers, which we demonstrated by analyzing phosphorylated H2A.X foci in zygonema. Using the seed-typing technique, we found that hotspot usage remains mainly unchanged in atr mutants compared with wild-type individuals. Moreover, atr showed no change in the number of crossovers caused by two independent pathways, which implies no effect on crossover pathway choice. Analyses of genetic interaction indicate that while the effects of atr are independent of MMS AND UV SENSITIVE81 (MUS81), ZIPPER1 (ZYP1), FANCONI ANEMIA COMPLEMENTATION GROUP M (FANCM), and D2 (FANCD2), the underlying mechanism may be similar between ATR and FANCD2. This study extends our understanding of ATR's role in meiosis, uncovering functions in regulating crossover distribution.engAttribution 4.0 Internationalhttp://creativecommons.org/licenses/by/4.0/journal article1532-298Xhttps://doi.org/10.1093/plcell/koae292https://academic.oup.com/plcell/advance-article/doi/10.1093/plcell/koae292/7848887open access58575577.2Botánica (Biología)GenéticaBiología molecular (Biología)2417 Biología Vegetal (Botánica)2417.14 Genética Vegetal2401.08 Genética Animal2409.92 Genética Molecular de Plantas2415 Biología Molecular