Foronda, DavidPérez-Garijo, AinhoaMartín Castro, Francisco Antonio2025-11-282025-11-282008-12-11Foronda D, Pérez-Garijo A, Martín FA. Dpp of posterior origin patterns the proximal region of the wing. Mech Dev. 2009 Mar-Apr;126(3-4):99-106. doi: 10.1016/j.mod.2008.12.002. Epub 2008 Dec 11. PMID: 19118625.0925-477310.1016/j.mod.2008.12.002https://hdl.handle.net/20.500.14352/127370Acknowledgments: We thank Ernesto Sanchez-Herrero and Gines Morata for continuous support throughout this work, Gines Morata for help with the writing, Carlos Estella, Hector Herranz, Lara Salvany, Luis María Escudero, Magali Suzanne, Joaquín Cul, Jose Felix de Celis and Ernesto Sanchez-Herrero for critical reading of the paper, Manuel Calleja for helping with Fig. 3, Rosa Gonzalez and Angelica Cantarero for general help and the Bloomington Stock Center, Tetsuya Tabata, Gerard Campbell and Andrea Tomlinson for fly stocks. This work was supported by Grants from the Ministerio de Educación y Ciencia and an Institutional Grant from the Fundación Ramón Areces.The decapentaplegic (dpp) gene encodes a long-range morphogen that plays a key role in the patterning of the wing imaginal disc of Drosophila (Nellen, D., Burke, R., Struhl, G. and Basler, K. 1996. Direct and long-range action of a DPP morphogen gradient. Cell 85, 357–368.). The current view is that dpp is transcriptionally active in a narrow band of anterior compartment cells close to the anterio-posterior (A/P) compartment border. Once the Dpp protein is synthesised, it travels across the A/P border and diffuses forming concentration gradients in the two compartments (reviewed in Lawrence, P.A., Struhl, G. 1996. Morphogens, compartments, and pattern: lessons from drosophila? Cell 85, 951–961.). We have found a new site of dpp expression in the posterior wing compartment which appears during the third larval period. This source of Dpp signal generates a local gradient of Dpp pathway activity, which is independent of that originating in the anterior compartment. We show that this posterior tier of Dpp activity is functionally required for normal wing development: the elimination of dpp expression in the posterior compartment results in defective adult wings in which pattern elements such as the alula and much of the axillary cord are not formed. Moreover, these structures develop normally in the absence of anterior dpp expression. Thus the normal wing pattern requires distinct Dpp organizer activities in the anterior and posterior compartments. We further show that, unlike the anterior dpp expression domain, the posterior one is not dependent on Hedgehog activity but is dependant on the activity of the IRO complex gene mirror. Since there is a similar expression in the haltere disc, we suggest that this late appearing posterior Dpp activity may be an attribute of dorsal thoracic discs.engjournal articlehttps://doi.org/10.1016/j.mod.2008.12.002open access575Dpp morphogenWing discCompartmentsPatterningBiología molecular (Biología)Biología celular (Biología)Biología2401.05 desarrollo Animal2407.01 Cultivo Celular2409 Genética2415.01 Biología Molecular de Microorganismos