Martínez De La Casa Fernández-Borrella, José MaríaPérez Bartolomé, FranciscoUrcelay, ElenaSantiago, José LuisMoreno Montañes, JavierArriola Villalobos, PedroBenítez Del Castillo Sánchez, José ManuelGarcía Feijoo, Julián2023-06-172023-06-172017-03Martínez De La Casa Fernández-Borrella, J. M., Pérez Bartolomé, F:, Urcelay, E. et al. «Tear Cytokine Profile of Glaucoma Patients Treated with Preservative-Free or Preserved Latanoprost». The Ocular Surface, vol. 15, n.o 4, octubre de 2017, pp. 723-29. DOI.org (Crossref), https://doi.org/10.1016/j.jtos.2017.03.004.1542-012410.1016/j.jtos.2017.03.004https://hdl.handle.net/20.500.14352/17803Purpose: To determine variations in cytokine levels of glaucoma patients treated either with preservative-free latanoprost or preserved latanoprost, relative to healthy individuals. Methods: Tear samples were collected from 39 healthy subjects, 20 glaucoma patients treated with preserved latanoprost, and 20 patients treated with preservative-free latanoprost. A set of 27 inflammatory cytokines was analyzed in each group, including interleukin (IL)-1β, IL-1ra, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL9, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, eotaxin, fibroblast growth factor (FGF) basic, granulocyte colony stimulating factor (G-CSF), granulocyte monocyte colony stimulating factor (GM-CSF), interferon (IFN)-γ, interferon gamma-induced protein (IP)-10, monocyte chemo attractant protein (MCP)-1MCAF, macrophage inflammatory protein (MIP)-1α, MIP-1β, platelet-derived growth factor (PDGF)-BB, regulated on activation, normal T cell expressed and secreted (RANTES), tumor necrosis factor (TNF)-α and vascular endothelial growth factor (VEGF). Cytokine concentrations were obtained by the Bio-Plex Human Cytokine Immunoassay. Non-invasive tear breakup time (NI-TBUT), tear meniscus height, corneal fluorescein staining, conjunctival hyperemia and ocular surface disease index (OSDI) were assessed in patients treated with preservative-free and preserved latanoprost. Results: The levels of IL-2, IL-5, IL-10, IL-12 (p70), IL-13, IL-15, IL-17, FGF basic, PDGF-BB, and TNF-α were significantly higher in patients receiving preserved latanoprost, compared to normal controls (p < 0.05). The expression of all the cytokines studied remained statistically invariable in patients receiving preservative-free latanoprost, compared to healthy subjects (p > 0.05). Ocular surface parameters were not significantly different in both glaucoma groups, and no correlation between these clinical parameters and cytokine levels was observed. Conclusions: Treatment with preserved latanoprost has a direct impact on tear cytokine levels, whereas this effect is not observed upon preservative-free latanoprost instillation.engAtribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/journal articlehttps://doi.org/10.1016/j.jtos.2017.03.004http://www.sciencedirect.com/science/article/pii/S1542012416302695https://www.ncbi.nlm.nih.gov/pubmed/28342814open access615.457617.7-007.681CytokineGlaucomaInflammationLatanoprostOcular surface diseasePreservativesOftalmologíaMedicamentos3201.09 Oftalmología