Sánchez Rodríguez, AnaArias Álvarez, MaríaMillán Pastor, María PilarLorenzo González, Pedro LuisGarcía García, Rosa MaríaGarcía Rebollar, Pilar2024-01-172024-01-172020Sanchez-Rodriguez, Ana, et al. «Physiological Effects on Rabbit Sperm and Reproductive Response to Recombinant Rabbit Beta Nerve Growth Factor Administered by Intravaginal Route in Rabbit Does». Theriogenology, vol. 157, noviembre de 2020, pp. 327-34. https://doi.org/10.1016/j.theriogenology.2020.08.003.0093-691X10.1016/j.theriogenology.2020.08.003https://hdl.handle.net/20.500.14352/93720Credit authorship contribution statement Ana Sanchez-Rodriguez: Conceptualization, Methodology, Formal analysis, Investigation, Writing - original draft. María Arias-Álvarez: Methodology, Resources, Writing - review & editing, Funding acquisition. Pilar Millán: Methodology, Resources, Writing - review & editing. Pedro L. Lorenzo: Conceptualization, Resources, Writing - review & editing, Supervision, Funding acquisition. Rosa M. García-García: Conceptualization, Methodology, Formal analysis, Investigation, Writing - review & editing, Supervision, Funding acquisition. Pilar G. Rebollar: Conceptualization, Methodology, Formal analysis, Investigation, Resources, Writing - review & editing, Supervision, Funding acquisition.Beta nerve growth factor (β-NGF) is present in the seminal plasma of some species, including rabbits, acting as an ovulation-inducing factor in camelids. Traditionally, GnRH analogues are used to induce ovulation by intramuscular route when artificial insemination (AI) is performed in rabbit does. A specific rabbit recombinant β-NGF (rrβ-NGF) produced in our laboratory was tested as an alternative method to conventional treatment with GnRH analogues to induce ovulation. In the present work, different concentrations (0, 20, 100 ng/mL and 1, 20 and 100 μg/mL) of rrβ-NGF were added to diluted semen to assess its effect on sperm traits (viability and motility parameters). rrβ-NG was used also, incorporated to the AI dose, to evaluate ovulation response (LH and progesterone plasma concentrations, ovulation rate (OR) and embryo implantation at Day 7) after intravaginal administration. A negative control group stimulated with an empty catheter, and a positive control group inseminated and intramuscularly treated as usual with GnRH were also set up. Results showed that seminal quality was influenced by rrβ-NGF depending on the concentration added, being the highest concentrations tested deleterious for semen. Whereas the highest OR was found in the positive control group (100%), concentrations of 20 ng/mL, 1 μg/mL and 20 μg/mL of rrβ-NGF triggered intermediate OR (30, 60 and 42.9%, respectively), and 100 ng/mL and 100 μg/mL had the lowest OR (20 and 14.3%, respectively). Although LH peak was not observed in the first 2 h after AI in the ovulated females from rrβ-NGF groups, plasma progesterone significantly increased at Day 7, except in those females treated with 20 and 100 μg/mL. Also, 98.4% of ovulated females were pregnant on Day 7. In conclusion, rrβ-NGF added to diluted semen affects seminal quality and provokes ovulation, the development of functional CL and conception by intravaginal route in rabbit does, depending on the concentration added.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal article1879-3231https://doi.org/10.1016/j.theriogenology.2020.08.003restricted access636.08Recombinant rabbit β NGFSemenOvulation inductionLH peakRabbitProgesteroneProducción animalFisiología veterinaria3104 Producción Animal2401.13 Fisiología Animal