Valverde de la Fuente, AlejandroGordón Pidal, José MaríaMontero Calle, AnaArévalo Pérez, BeatrizSerafín González-Carrato, VerónicaCalero, MiguelMoreno Guzmán, MaríaLópez, Miguel ÁngelEscarpa, AlbertoYáñez Sedeño, PalomaBarderas, RodrigoCampuzano Ruiz, SusanaPingarrón Carrazón, José Manuel2023-06-222023-06-222022-02-012196-021610.1002/celc.202200055https://hdl.handle.net/20.500.14352/71373CRUE-CSIC (Acuerdos Transformativos 2022)Alzheimer’s disease (AD), the most common neurodegenerative disorder, demands new cost-effective and easy-to-use strategies for its reliable detection, mainly in the preclinical stages. Here, we report the first immunoplatform for the electrochemical multidetermination of four candidate protein biomarkers in blood, neurofilament light chain (NfL), Tau, phosphorylated Tau (p-Tau) and TAR DNA-Binding Protein 43 (TDP-43). It involves implementation of sandwich-type immunoassays and enzymatic labelling with horseradish peroxidase (HRP) on the surface of magnetic microbeads (MBs). Amperometric detection is performed after depositing the magnetic immunoconjugates on disposable quadruple transduction platforms by monitoring the enzymatic reduction of H2O2 mediated by hydroquinone (HQ). The immunoplatform achieved LOD values smaller than the content of target biomarkers in plasma of healthy subjects, with RSD values.engAtribución-NoComercial 3.0 Españajournal articlehttps://doi.org/10.1002/celc.202200055open accessAlzheimer’s diseaseCandidate biomarkersElectrochemicalmultiplexing immunoplatformMagnetic microbeadsPlasmaQuímicaQuímica analítica (Química)Neurociencias (Medicina)23 Química2301 Química Analítica2490 Neurociencias