Gil-Serna, JessicaPatiño Álvarez, Aurora BelénJurado, MiguelMirete Castañeda, SalvadorVázquez Estévez, CovadogaGonzález Jaén, María Teresa2023-06-182023-06-182017978-1-4939-6705-6 (Print) 978-1-4939-6707-0 (Online)10.1007/978-1-4939-6707-0_8https://hdl.handle.net/20.500.14352/19456Fumonisins are important mycotoxins contaminating foods and feeds which are mainly produced by F. verticillioides and F. proliferatum. Additionally, both are pathogens of maize and other cereals. We describe two highly sensitive, rapid, and species-specific PCR protocols which enable detection and discrimination of these closely related species in cereal flour or grain samples. The specific primer pairs of these assays were based on the intergenic spacer region of the multicopy rDNA unit which highly improves the sensitivity of the PCR assay in comparison with single-copy target regions.engbook parthttps://link.springer.com/protocol/10.1007%2F978-1-4939-6707-0_8restricted access575582.282Fusarium verticillioidesFusarium proliferatumFumonisinsPolymerase chain reaction (PCR)DetectionrDNA intergenic spacer (IGS)BiologíaGenéticaMicrobiología (Biología)24 Ciencias de la Vida2409 Genética2414 Microbiología