Sanz Santamaría, Ana BelénGarcía Sánchez, RaúlRodríguez Peña, José ManuelDíez Muñiz, SoniaNombela Cano, CésarPeterson, Craig L.Arroyo Nombela, Francisco Javier2023-06-202023-06-202012-05-231939-458610.1091/mbc.E12-04-0278https://hdl.handle.net/20.500.14352/43872In Saccharomyces cerevisiae, the transcriptional program triggered by cell wall stress is coordinated by Slt2/Mpk1, the mitogen-activated protein kinase (MAPK) of the cell wall integrity (CWI) pathway, and is mostly mediated by the transcription factor Rlm1. Here we show that the SWI/SNF chromatin-remodeling complex plays a critical role in orchestrating the transcriptional response regulated by Rlm1. swi/snf mutants show drastically reduced expression of cell wall stress-responsive genes and hypersensitivity to cell wall-interfering compounds. On stress, binding of RNA Pol II to the promoters of these genes depends on Rlm1, Slt2, and SWI/SNF. Rlm1 physically interacts with SWI/SNF to direct its association to target promoters. Finally, we observe nucleosome displacement at the CWI-responsive gene MLP1/KDX1, which relies on the SWI/SNF complex. Taken together, our results identify the SWI/SNF complex as a key element of the CWI MAPK pathway that mediates the chromatin remodeling necessary for adequate transcriptional response to cell wall stress.engAttribution-NonCommercial-ShareAlike 4.0 Internationalhttps://creativecommons.org/licenses/by-nc-sa/4.0/journal articlehttp://www.molbiolcell.org/content/23/14/2805open access579MicrobiologíaMicrobiología (Farmacia)3302.03 Microbiología Industrial