Torres, VivianaHamdi, MeriemMaillo, VerónicaUrrego, RodrigoEcheverri, Jose JulianLópez Herrera, AlbeiroGutiérrez Adán, AlfonsoRizos, DimitriosSánchez Calabuig, María Jesús2025-02-052025-02-052019Torres, V., Hamdi, M., Maillo, V., Urrego, R., Echeverri, J. J., López-Herrera, A., Gutiérrez-Adán, A., Rizos, D., & Sánchez-Calabuig, M. J. (2019). Ascorbic acid–cyclodextrin complex alters the expression of genes associated with lipid metabolism in bovine in vitro produced embryos. Reproduction in Domestic Animals, 54(1), 55-62. https://doi.org/10.1111/RDA.133110936-676810.1111/rda.13311https://hdl.handle.net/20.500.14352/117847Authors Contributions: A Gutiérrez‐Adán, D Rizos and MJ Sánchez‐Calabuig designed the experiment. V Torres, M Hamdi M, Maillo V and MJ Sánchez‐ Calabuig performed the experiment and analysed data. V Torres and MJ Sánchez‐Calabuig drafted the paper. R Urrego, J Echeverri and A López‐Herrera contributed with the experiment design and the paper corrections.Ascorbic acid (AC) used as antioxidant in embryo culture is very sensitive and degrades unavoidably in aqueous solution. Methyl-β-cyclodextrin (CD) improved the stability of AC in solution to elevated temperature, light, humidity and oxidation. The aim of this study was to evaluate the effect of the complex AC-CD during in vitro maturation (IVM) or in vitro culture (IVC) on oocyte developmental competence and subsequent embryo development and quality. AC-CD (100 µM) was added to IVM media, and maturation level and embryo development were examined. Matured oocytes, their cumulus cells and produced blastocysts were snap-frozen for gene expression analysis by RT-qPCR. Besides, in vitro-produced zygotes were cultured with 100 µM of AC-CD and blastocysts were as well snap-frozen for gene expression analysis. A group without AC-CD (control- ) and other with CD (control+ ) were included. No differences were found on maturation, cleavage or blastocyst rates. However, in matured oocytes, AC-CD downregulated BAX, GPX1 and BMP15. In cumulus cells, AC-CD downregulated BAX/BCL2 and GSTA4 while upregulated BCL2 and CYP51A1. The expression of SL2A1, FADS1, PNPLA and MTORC1 was downregulated in blastocysts derived from oocytes matured with AC-CD, while in blastocysts derived from zygote cultured with AC-CD, CYP51A1 and IGF2R were downregulated and PNPLA2 was upregulated. In conclusion, AC-CD in both IVM and IVC media may reduce accumulated fat by increasing lipolysis and suppressing lipogenesis in blastocysts derived from both oocytes and zygotes cultured with AC-CD, suggesting that CD improves the quality of embryos and bioavailability of AC during IVM and IVC.engjournal article1439-0531https://doi.org/10.1111/rda.1331130120843restricted access636.082.4Ascorbic acidEmbryo developmentGene expressionMethyl‐β‐cyclodextrinFisiología animal (Biología)3104.11 Reproducción