Carrasco Padilla, CAguilar Sopeña, OGómez Morón, AlvaroAlegre Gómez, SergioSánchez Madrid, FranciscoMartín Cófreces, Noa BeatrizRoda Navarro, Pedro2025-05-132025-05-132023Carrasco-Padilla C, Aguilar-Sopeña O, Gómez-Morón A, Alegre-Gómez S, Sánchez-Madrid F, Martín-Cófreces NB, Roda-Navarro P. T cell activation and effector function in the human Jurkat T cell model. Methods Cell Biol. 2023;178:25-41. doi: 10.1016/bs.mcb.2022.09.012. Epub 2022 Nov 12.10.1016/bs.mcb.2022.09.012https://hdl.handle.net/20.500.14352/120023In order to understand T cell function, it is necessary to completely decipher the molecular dynamics underlying T cell activation and effector function. In vitro easy-to-handle cellular models are valuable tools to study intracellular molecular mechanisms in live cells. The CD4 T cell line Jurkat (JK) has been widely employed to investigate intracellular signaling leading to T cell activation in response to T cell receptor (TCR) triggering. Here, we describe diverse, complementary protocols to evaluate the TCR- and costimulation-mediated T cell activation, as well as the immunological synapse assembly and cytokine production occurring as a consequence of successful early activation events. This in vitro model is extremely useful to address molecular mechanisms operating during T cell activation and effector function acting in diverse pathophysiological scenarios.engbook parthttps://doi.org/10.1016/bs.mcb.2022.09.01237516527open access616.079Confocal microscopyImmunological synapseTotal internal reflexion fluorescence microscopyCiencias BiomédicasInmunologíaBiología celular (Biología)32 Ciencias Médicas2412 Inmunología