Khodarahmi, RezaTorrente Rodríguez, Rebeca MagnoliaRuiz-Valdepeñas Montiel, VíctorCampuzano Ruiz, SusanaPedrero Muñoz, MaríaFarchado, MeryemVargas, EvaManuel De Villena Rueda, Francisco JavierGarranzo Asensio, MaríaBarderas Manchado, RodrigoPingarrón Carrazón, José Manuel2023-06-172023-06-1720171932-620310.1371/journal.pone.0175056https://hdl.handle.net/20.500.14352/18048The first electrochemical immunosensor for the determination of fibroblast growth factor receptor 4 (FGFR4) biomarker is reported in this work. The biosensor involves a sandwich configuration with covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic microcarriers (HOOC-MBs) and amperometric detection at disposable carbon screen-printed electrodes (SPCEs). The biosensor exhibits a great analytical performance regarding selectivity for the target protein and a low LOD of 48.2 pg mL-1. The electrochemical platform was successfully applied for the determination of FGFR4 in different cancer cell lysates without any apparent matrix effect after a simple sample dilution and using only 2.5 μg of the raw lysate. Comparison of the results with those provided by a commercial ELISA kit shows competitive advantages by using the developed immunosensor in terms of simplicity, analysis time, and portability and cost-affordability of the required instrumentation for the accurate determination of FGFR4 in cell lysates.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.1371/journal.pone.0175056open access543Química analítica (Química)2301 Química Analítica