Amorim, Adriany das Graças NascimentoSánchez-Paniagua López, MartaOliveira, Taiane Maria deMafud, Ana CarolinaAlves da Silva, DurcileneSouza de Almeida Leite, José Roberto deLópez Ruiz, María Beatriz2024-02-072024-02-072021-08-02Das Graças Nascimento Amorim A, Sánchez-Paniagua M, De Oliveira TM, Mafud AC, Da Silva DA, De Souza De Almeida Leite JR, et al. Synthesis, characterization and use of enzyme cashew gum nanoparticles for biosensing applications. J Mater Chem B 2021;9:6825–35. https://doi.org/10.1039/D1TB01164B.2050-750X10.1039/d1tb01164bhttps://hdl.handle.net/20.500.14352/99976This research reports, for the first time, the immobilization of an enzyme - Rhus vernificera laccase - on cashew gum (CG) nanoparticles (NPs) and its application as a biological layer in the design and development of an electrochemical biosensor. Laccase-CG nanoparticles (LacCG-NPs) were prepared by the nanoprecipitation method and characterized by UV-Vis spectrophotometry, atomic force microscopy, scanning electron microscopy, attenuated total reflectance-Fourier-transform infrared spectroscopy, circular dichroism, cyclic voltammetry, and electrochemical impedance spectroscopy. The average size and stability of the NPs were predicted by DLS and zeta potential. The ATR-FTIR results clearly demonstrated an interaction between –NH and –OH groups to form LacCG-NPs. The average size found for LacCG-NPs was 280 53 nm and a polydispersity index of 0.309 0.08 indicated a good particle size distribution. The zeta potential shows a good colloidal stability. The use of a natural product to prepare the enzymatic nanoparticles, its easy synthesis and the immobilization efficiency should be highlighted. LacCG-NPs were successfully applied as a biolayer in the development of an amperometric biosensor for catechol detection. The resulting device showed a low response time (6 s), good sensitivity (7.86 mA mM1 cm2 ), wide linear range of 2.5 107 –2.0 104 M, and low detection limit (50 nM).engjournal article2050-7518https://doi.org/10.1039/d1tb01164brestricted accessQuímica analítica (Química)2301 Química Analítica