Almendro Vedia, Víctor GalileoNatale, PaoloMell, MichaelBonneau, StephanieMonroy Muñoz, FranciscoJoubert, FredericLópez-Montero, Iván2023-06-172023-06-1720170027-842410.1073/pnas.1701207114https://hdl.handle.net/20.500.14352/19021The research leading to these results has received funding from the European Research Council under the European Union's Seventh Framework Programme (ERC grant agreement n° 338133)ATP synthase is a rotating membrane protein that synthesizes ATP through proton-pumping activity across the membrane. To unveil the mechanical impact of this molecular active pump on the bending properties of its lipid environment, we have functionally reconstituted the ATP synthase in giant unilamellar vesicles and tracked the membrane fluctuations by means of flickering spectroscopy. We find that ATP synthase rotates at a frequency of about 20 Hz, promoting large nonequilibrium deformations at discrete hot spots in lipid vesicles and thus inducing an overall membrane softening. The enhanced nonequilibrium fluctuations are compatible with an accumulation of active proteins at highly curved membrane sites through a curvature−protein coupling mechanism that supports the emergence of collective effects of rotating ATP synthases in lipid membranes.engNonequilibrium fluctuations of lipid membranes by the rotating motor protein F1F0-ATP synthasejournal articlehttps://doi.org/10.1073/pnas.1701207114open accessGiant vesiclesactive membranesmechanical propertiesflickering spectroscopybiological nanorotorsQuímica física (Química)Bioquímica (Biología)FísicaBiofísica2302 Bioquímica