Barradas, MartaPlaza, AdriánColmenarejo, GonzaloLázaro, IolandaCosta-Machado, Luis FilipeMartín-Hernández, RobertoMicó, VictorLópez-Aceituno, José LuisHerranz, JesúsPantoja, CristinaTejero, HectorDiaz-Ruiz, AlbertoAl-Shahrour, FatimaDaimiel, LidiaLoria Kohen, Viviana ConstanzaRamírez de Molina, AnaEfeyan, AlejoSerrano, ManuelPozo, Oscar J.Sala-Vila, AleixFernández-Marcos, Pablo J.Springer Science and Business Media LLC2024-05-162024-05-162022-09-27Barradas M, Plaza A, Colmenarejo G, Lázaro I, Costa-Machado LF, Martín-Hernández R, Micó V, López-Aceituno JL, Herranz J, Pantoja C, Tejero H, Diaz-Ruiz A, Al-Shahrour F, Daimiel L, Loria-Kohen V, Ramirez de Molina A, Efeyan A, Serrano M, Pozo OJ, Sala-Vila A, Fernandez-Marcos PJ. Fatty acids homeostasis during fasting predicts protection from chemotherapy toxicity. Nat Commun. 2022 Sep 27;13(1):5677.2041-172310.1038/s41467-022-33352-3https://hdl.handle.net/20.500.14352/104101Fasting exerts beneficial effects in mice and humans, including protection from chemotherapy toxicity. To explore the involved mechanisms, we collect blood from humans and mice before and after 36 or 24 hours of fasting, respectively, and measure lipid composition of erythrocyte membranes, circulating micro RNAs (miRNAs), and RNA expression at peripheral blood mononuclear cells (PBMCs). Fasting coordinately affects the proportion of polyunsaturated versus saturated and monounsaturated fatty acids at the erythrocyte membrane; and reduces the expression of insulin signaling-related genes in PBMCs. When fasted for 24 hours before and 24 hours after administration of oxaliplatin or doxorubicin, mice show a strong protection from toxicity in several tissues. Erythrocyte membrane lipids and PBMC gene expression define two separate groups of individuals that accurately predict a differential protection from chemotherapy toxicity, with important clinical implications. Our results reveal a mechanism of fasting associated with lipid homeostasis, and provide biomarkers of fasting to predict fasting-mediated protection from chemotherapy toxicity.engjournal articlehttps://www.nature.com/articles/s41467-022-33352-3open access612.39BiologíaMedicinaDietética y nutrición (Farmacia)2410 Biología Humana2499 Otras Especialidades Biológicas