Martínez Caballero, María AranzazuAres Lombán, IrmaMartínez Caballero, MartaLópez Torres, BernardoRodríguez, José LuisMaximiliano Guerra, Jorge EnriqueMartínez Larrañaga, María RosaAnadón Navarro, Arturo RamónRosa, José Manuel de laCueto, Mercedes2024-02-012024-02-012020Martínez MA, Ares I, Martínez M, Lopez-Torres B, Rodríguez JL, Maximiliano JE, Martínez-Larrañaga MR, Anadón A, Rosa JM, Cueto M. Protective effects of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) against oxidant-induced stress in human colon carcinoma Caco-2 cells. Food Chem Toxicol. 2020 Nov;145:111671. doi: 10.1016/j.fct.2020.111671. Epub 2020 Aug 9. PMID: 32784000.0278-691510.1016/j.fct.2020.111671https://hdl.handle.net/20.500.14352/97616Credit authorship contribution statement María-Aránzazu Martínez: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation, Writing - review & editing. Irma Ares: Investigation, Formal analysis, Writing - original draft, Writing - review & editing. Marta Martínez: Investigation, Formal analysis, Writing - original draft, Writing - review & editing. Bernardo Lopez-Torres: Methodology, Investigation. Jose-Luis Rodríguez: Methodology, Investigation, Formal analysis. Jorge-Enrique Maximiliano: Methodology, Investigation. María-Rosa Martínez-Larrañaga: Conceptualization, Methodology, Investigation, Formal analysis. Arturo Anadón: Conceptualization, Methodology, Validation, Formal analysis, Investigation. Jose-Manuel de la Rosa: Methodology, Investigation. Mercedes Cueto: Conceptualization, Funding acquisition, Methodology, Validation, Formal analysis, Investigation, Writing - review & editing.The present study investigated the effect of culture extracts (CB08035-SCA and CB08035-SYP) from Marinobacter hydrocarbonoclasticus (strain CB08035) on cell viability and the potential protective effects attributed to molecular mechanisms underlying antioxidant response to survive oxidative stress injuries. Caco-2 cells were submitted to oxidative stress by treatment with tert-butyl hydroperoxide (t-BOOH). Both extracts prevented cell damage and enhanced activity of antioxidant defenses (NQO1 and GST activities and GSH levels) reduced by treatment with t-BOOH. Increased ROS and caspase 3/7 activity induced by t-BOOH were dose-dependently prevented when cells were treated with the extracts. CB08035-SCA caused up-regulation of Nrf2, AKT1 and Bcl-2 gene expressions. Moreover, CB08035-SCA and CB08035-SYP treatments reduced significantly Bax, BNIP3, APAF1, ERK1, JNK1, MAPK1, NFκB1, TNFα, IL-6, IL-1β and HO-1 gene expressions of apoptosis, proinflammation and oxidative stress induced by t-BOOH. CB08035-SCA and CB08035-SYP CPE extracts confer a significant protection against oxidative insults to cells. Our results show that culture extracts CB08035-SCA and CB08035-SYP from M. hydrocarbonoclasticus (strain CB08035) appeared to have antioxidant potential, based on their ability to protect antioxidant enzymes and mRNA gene expressions linked to apoptosis/oxidative pathways. These results suggest that culture extracts CB08035-SCA and CB08035-SYP can be a potential ingredient in the pharmaceutical and cosmeceutical industries.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://doi.org/10.1016/j.fct.2020.11167132784000restricted access613.2.099636.09Marinobacter hydrocarbonoclasticus (Strain CB08035)CB08035-SCA and CB08035-SYP extractsPhenyl-acetic derivativesOxidative stressCaco-2 cellsVeterinaria3214 Toxicología