Ortiz Sánchez, PaulaSara González-SotoLuz H VillamizarValencia Mahón, JarisJiménez Pérez, EvaSacedón Ayuso, RosaManuel RamírezIsabel FariñasVaras Fajardo, AlbertoMartínez Fernández De Sevilla, LidiaVicente López, María Ángeles2026-01-132026-01-132025-02-12Ortiz-Sánchez, P., González-Soto, S., Villamizar, L. H., Valencia, J., Jiménez, E., Sacedón, R., Ramírez, M., Fariñas, I., Varas, A., Fernández-Sevilla, L. M., & Vicente, Á. (2025). Meningeal leukaemic aggregates as foci of cell expansion and chemoresistance in acute lymphoblastic leukaemia metastasis. Cellular Oncology, 48(3), 725-741. https://doi.org/10.1007/S13402-025-01043-Y10.1007/s13402-025-01043-yhttps://hdl.handle.net/20.500.14352/129997Purpose: Central nervous system (CNS) involvement and/or relapse remains one of the most important causes of morbidity/mortality in paediatric B-cell precursor acute lymphoblastic leukaemia (BCP-ALL) patients. To identify novel therapeutic targets and develop less aggressive therapies, a better understanding of the cellular and molecular microenvironment in leptomeningeal metastases is key. Here, we aimed to investigate the formation of metastatic leptomeningeal aggregates and their relevance to the expansion, survival and chemoresistance acquisition of leukaemia cells. Methods: We used BCP-ALL xenograft mouse models, combined with immunohistofluorescence and flow cytometry, to study the development of CNS metastasis and the contribution of leptomeningeal cells to the organisation of leukaemic aggregates. To in vitro mimic the CNS metastasis, we established co-cultures of three-dimensional (3D) ALL cell spheroids and human leptomeningeal cells (hLMCs) and studied the effects on gene expression, proliferation, cytokine production, and chemoresistance. Results: In xenografted mice, ALL cells infiltrated the CNS at an early stage and, after crossing an ER-TR7+ fibroblast-like meningeal cell layer, they proliferated extensively and formed large vascularised leukaemic aggregates supported by a network of podoplanin+ leptomeningeal cells. In leukaemia spheroid-hLMC co-cultures, unlike conventional 2D co-cultures, meningeal cells strongly promoted the proliferation of leukaemic cells and generated a pro-inflammatory microenvironment. Furthermore, in 3D cell aggregates, leukaemic cells also developed chemoresistance, at least in part due to ABC transporter up-regulation. Conclusion: Our results provide evidence for the formation of metastatic ALL-leptomeningeal cell aggregates, their pro-inflammatory profile and their contribution to leukaemic cell expansion, survival and chemoresistance in the CNS.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/journal articlehttps://link.springer.com/article/10.1007/s13402-025-01043-yhttps://pubmed.ncbi.nlm.nih.gov/39937211/#full-view-affiliation-6open access616.43D cell spheroidsAcute lymphoblastic leukaemiaCell expansionCentral nervous systemChemoresistanceMeningeal cellsInmunologíaOncologíaBiología celular (Biología)Histología2407 Biología Celular2412 Inmunología3207.13 Oncología3201.01 Oncología