Cloning and heterologous production of hiracin JM79, a sec-dependent bacteriocin produced by enterococcus hirae DCH5, in lactic acid bacteria and pichia pastoris

Sánchez, JorgeBorrero Del Pino, JuanGómez Sala, BeatrizBasanta, AntonioHerranz Sorribes, CarmenCintas Izarra, Luis MiguelHernández Cruza, Pablo Elpidio2025-01-202025-01-202008-04-15Sánchez, J., Borrero, J., Gómez-Sala, B., Basanta, A., Herranz, C., Cintas, L. M., & Hernández, P. E. (2008). Cloning and heterologous production of hiracin JM79, a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, in lactic acid bacteria and Pichia pastoris. Applied and Environmental Microbiology, 74(8), 2471-2479. https://doi.org/10.1128/AEM.02559-070099-22401098-533610.1128/aem.02559-07https://hdl.handle.net/20.500.14352/115213Hiracin JM79 (HirJM79), a Sec-dependent bacteriocin produced by Enterococcus hirae DCH5, was cloned and produced in Lactococcus lactis, Lactobacillus sakei, Enterococcus faecium, Enterococcus faecalis, and Pichia pastoris. For heterologous production of HirJM79 in lactic acid bacteria (LAB), the HirJM79 structural gene (hirJM79), with or without the HirJM79 immunity gene (hiriJM79), was cloned into the plasmid pMG36c under the control of the constitutive promoter P32 and into the plasmid pNZ8048 under the control of the inducible PNisA promoter. For the production of HirJM79 in P. pastoris, the gene encoding the mature HirJM79 protein was cloned into the pPICZαA expression vector. The recombinant plasmids permitted the production of biologically active HirJM79 in the supernatants of L. lactis IL1403, L. lactis NZ9000, L. sakei Lb790, E. faecalis JH2-2, and P. pastoris X-33, the coproduction of HirJM79 and nisin A in L. lactis DPC5598, and the coproduction of HirJM79 and enterocin P in E. faecium L50/14-2. All recombinant LAB produced larger quantities of HirJM79 than E. hirae DCH5, although the antimicrobial activities of most transformants were lower than that predicted from their production of HirJM79. The synthesis, processing, and secretion of HirJM79 proceed efficiently in recombinant LAB strains and P. pastoris. Copyright © 2008, American Society for Microbiology.engAttribution-NonCommercial-NoDerivatives 4.0 Internationalhttp://creativecommons.org/licenses/by-nc-nd/4.0/Cloning and heterologous production of hiracin JM79, a sec-dependent bacteriocin produced by enterococcus hirae DCH5, in lactic acid bacteria and pichia pastorisjournal articlehttps://doi.org/10.1128/AEM.02559-07open accessMicrobiología (Veterinaria)3109 Ciencias Veterinarias