Gil Dones, FélixBrun, AlejandroWigdorovitz, AndrésCatalá, RafaelMartı́nez-Torrecuadrada, JorgeCasal, IgnacioSalinas, JulioBorca, ManuelEscribano, José2024-01-222024-01-222001Gil, Félix, et al. «High‐yield Expression of a Viral Peptide Vaccine in Transgenic Plants». FEBS Letters, vol. 488, n.o 1-2, enero de 2001, pp. 13-17. https://doi.org/10.1016/S0014-5793(00)02405-4.0014-579310.1016/s0014-5793(00)02405-4https://hdl.handle.net/20.500.14352/94486This work was supported by Grant 07B/0014/99 from Comunidad Autónoma de Madrid and by Grant BID802/OC-AR PID168 from SECYT-CONICET.A high-yield production of a peptide vaccine in transgenic plants is described here. A 21-mer peptide, which confers protection to dogs against challenge with virulent canine parvovirus, has been expressed in transgenic plants as an amino-terminal translational fusion with the GUS gene. Transformants were selected on the basis of their GUS activities, showing expression levels of the recombinant protein up to 3% of the total leaf soluble protein, a production yield comparable to that obtained with the same epitope expressed by chimeric plant viruses. The immunogenicity of the plant-derived peptide was demonstrated in mice immunized either intraperitoneally or orally with transgenic plant extracts, providing the suitability of the GUS fusions approach for low-cost production of peptide vaccines.engjournal article1873-3468https://doi.org/10.1016/s0014-5793(00)02405-4open access581.15Peptide vaccineCanine parvovirusTransgenic plantFusion proteinGenéticaBotánica (Biología)2417.14 Genética Vegetal2417 Biología Vegetal (Botánica)