Pedrero Muñoz, MaríaManuel De Villena Rueda, Francisco JavierMuñoz San Martín, CristinaCampuzano Ruiz, SusanaGarranzo Asensio, MaríaBarderas Manchado, RodrigoPingarrón Carrazón, José Manuel2023-06-182023-06-182016-11-212079-637410.3390/bios6040056https://hdl.handle.net/20.500.14352/19176An amperometric magnetoimmunosensor for the determination of human p53 protein is described in this work using a sandwich configuration involving the covalent immobilization of a specific capture antibody onto activated carboxylic-modified magnetic beads (HOOC-MBs) and incubation of the modified MBs with a mixture of the target protein and horseradish peroxidase-labeled antibody (HRP-anti-p53). The resulting modified MBs are captured by a magnet placed under the surface of a disposable carbon screen-printed electrode (SPCE) and the amperometric responses are measured at −0.20 V (vs. an Ag pseudo-reference electrode), upon addition of hydroquinone (HQ) as a redox mediator and H2O2 as the enzyme substrate. The magnetoimmunosensing platform was successfully applied for the detection of p53 protein in different cell lysates without any matrix effect after a simple sample dilution. The results correlated accurately with those provided by a commercial ELISA kit, thus confirming the immunosensor as an attractive alternative for rapid and simple determination of this protein using portable and affordable instrumentation.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/journal articlehttps://doi.org/10.3390/bios6040056https://www.mdpi.com/2079-6374/6/4/56open accesshuman p53magnetic microcarriersscreen-printed electrodesamperometric immunosensorcell lysatesBioquímica (Química)Química analítica (Química)2301 Química Analítica