Robles-Vera, IñakiCallejo Arranz, MaríaRamos, RicardoDuarte, JuanPérez Vizcaíno, Francisco2023-06-172023-06-172019-10-242072-664310.3390/nu11112564https://hdl.handle.net/20.500.14352/8205Inadequate immunologic, metabolic and cardiovascular homeostasis has been related to either an alteration of the gut microbiota or to vitamin D deficiency. We analyzed whether vitamin D deficiency alters rat gut microbiota. Male Wistar rats were fed a standard or a vitamin D-free diet for seven weeks. The microbiome composition was determined in fecal samples by 16S rRNA gene sequencing. The vitamin D-free diet produced mild changes on α- diversity but no effect on β-diversity in the global microbiome. Markers of gut dysbiosis like Firmicutes-to-Bacteroidetes ratio or the short chain fatty acid producing bacterial genera were not significantly affected by vitamin D deficiency. Notably, there was an increase in the relative abundance of the Enterobacteriaceae, with significant rises in its associated genera Escherichia, Candidatus blochmannia and Enterobacter in vitamin D deficient rats. Prevotella and Actinomyces were also increased and Odoribacteraceae and its genus Butyricimonas were decreased in rats with vitamin D-free diet. In conclusion, vitamin D deficit does not induce gut dysbiosis but produces some specific changes in bacterial taxa, which may play a pathophysiological role in the immunologic dysregulation associated with this hypovitaminosis.engAtribución 3.0 Españahttps://creativecommons.org/licenses/by/3.0/es/Impact of Vitamin D Deficit on the Rat Gut Microbiomejournal articlehttps://doi.org/10.3390/nu11112564https://www.mdpi.com/2072-6643/11/11/2564open accessmicrobiota16S rRNA sequencingvitamin D deficitMicrobiología médicaToxicología (Medicina)Animales de laboratorio3201.03 Microbiología Clínica3214 Toxicología