Pérez Sancho, MartaGarcía-Seco Romero, María TeresaArrogante, LGarcía, NGarcía Benzaquén, NereaMartínez Alares, IreneDíez Guerrier, Alberto AntoinePerales, AGoyache Goñi, JoaquínDomínguez Rodríguez, Lucas JoséÁlvarez Sánchez, Julio2024-01-162024-01-162013-10Pérez-Sancho, M., García-Seco, T., Arrogante, L., García, N., Martínez, I., Diez-Guerrier, A., Perales, A., Goyache, J., Domínguez, L., & Alvarez, J. (2013). Development and evaluation of an IS711-based loop mediated isothermal amplification method (LAMP) for detection of Brucella spp. on clinical samples. Research in veterinary science, 95(2), 489–494. https://doi.org/10.1016/j.rvsc.2013.05.0020034-528810.1016/j.rvsc.2013.05.002https://hdl.handle.net/20.500.14352/933912013 Elsevier Ltd. All rights reserved; Financiación: AGL-2009-12674 (Ministerio de Ciencia e Innovación). Consejería de Educación de la Comunidad de Madrid y Fondo Social Europeo. EU FP7 ANTIGONE Project Number 278976.DNA-based methods have emerged as an additional tool for Brucella infection-confirmation at a herd level. However, their implementation may require the use of specialized equipment. In this context the recently developed loop-mediated isothermal amplification (LAMP) technique may constitute an additional and cost-effective tool for rapid and specific DNA detection, especially in low income areas. In the present study the usefulness of a newly developed LAMP assay aiming at the multicopy-IS711 sequence was assessed on a variety of clinical samples (n = 81 from abortions and ewes; cattle, n = 3; swine, n = 4) that were analyzed in parallel using real-time PCR and bacteriology. Although overall sensitivities obtained with the three methods were comparable (p > 0.05), our results highlighted the complementarity between bacteriology and molecular-based methods for increased sensitivity. Significant differences (p < 0.05) were observed with all techniques depending on the nature of the sample. Our results demonstrate the potential of the IS711-LAMP technique for direct Brucella detection.engAttribution-NonCommercial-NoDerivatives 4.0 Internationaljournal article1532-2661https://www.sciencedirect.com/science/article/pii/S0034528813001811?via%3Dihubrestricted access636.09Bacteriology; Brucella; Loop mediated isothermal amplification; (LAMP); PCRVeterinariaSanidad animal3109 Ciencias Veterinarias