Garcia-Oliveira, Ana LuísaMartins-Lopesa, PaulaTolrác, RoserPoschenrieder, CharlotteTarquis, MartaGuedes-Pinto, HenriqueBenito Jiménez, César2024-10-012024-10-012014Garcia‐Oliveira, Ana Luísa, et al. «Molecular Characterization of the Citrate Transporter Gene TaMATE1 and Expression Analysis of Upstream Genes Involved in Organic Acid Transport under Al Stress in Bread Wheat ( Triticum Aestivum )». Physiologia Plantarum, vol. 152, n.o 3, noviembre de 2014, pp. 441-52. DOI.org (Crossref), https://doi.org/10.1111/ppl.12179.0031-931710.1111/ppl.12179https://hdl.handle.net/20.500.14352/108499Acknowledge the Foundation for Science and Technology (FCT), Portugal, for financial support to Ana Luísa Garcia-Oliveira (ALGO)(SFRH/BD/45556/2008).In bread wheat, besides malate, the importance of citrate efflux for Al tolerancehas also been reported. For better understanding the Al tolerance mechanismin bread wheat, here, we performed both a molecular characterization of thecitrate transporter gene TaMATE1 and an investigation on the upstream vari-ations in citrate and malate transporter genes. TaMATE1 belong to multidrugtransporter protein family, which are located on the long arm of homoeologousgroup 4 chromosomes (TaMATE1-4A, TaMATE1-4B TaMATE1-4D). TaMATE1homoeologues transcript expression study exhibited the preponderance ofhomoeologue TaMATE1-4B followed by TaMATE1-4D whereas homoeologueTaMATE1-4A seemed to be silenced. TaMATE1, particularly homoeologueTaMATE1-4B and TaALMT1 transcripts were much more expressed in the rootapices than in shoots of Al tolerant genotype Barbela 7/72/92 under both con-trol and Al stress conditions. In addition, in both tissues of Barbela 7/72/92,higher basal levels of these gene transcripts were observed than in Anahuac(Al sensitive). Noticeably, the presence of a transposon in the upstream ofTaMATE1-4B in Barbela 7/72/92 seems to be responsible for its higher tran-script expression where it may confer citrate efflux. Thus, promoter variations(transposon in TaMATE1-4B upstream and type VI promoter in TaALMT1) asso-ciated with higher basal transcript expression of TaMATE1-4B and TaALMT1clearly show how different mechanisms for Al tolerance operate simultane-ously in a single genotype. In conclusion, our results demonstrate that Barbela7/72/92 has favorable alleles for these organic acids transporter genes whichcould be utilized through genomic assisted selection to develop improvedcultivars for acidic soils.Abbreviations – 2D, two-dimensional; AACT, aluminum-activated citrate transporter; ALMT, Al-activated malate transporter;dNTP, deoxyribonucleotide triphosphates; EST, expressed sequence tag; MATE, multidrug and toxic compound exudation;OAs, organic acids; PCR, polymerase chain reaction; RT, reverse transcription; STOP, sensitive to proton rhizotoxicity.Physiol. Plant. 152, 2014 441engjournal article1399-3054https://doi.org/10.1111/ppl.12179https://onlinelibrary.wiley.com/doi/epdf/10.1111/ppl.12179restricted access581.1633.11664.6/.7Botánica (Biología)Fisiología vegetal (Biología)Alimentación2417 Biología Vegetal (Botánica)3309.07 Productos de Cereales3309.04 Panadería2409.92 Genética Molecular de Plantas