Ortiz Jaraba, IsabelDorado Martín, JesúsMorrell, Jane MCrespo Castejón, FranciscoGosálvez Berenguer, JaimeGálvez, MJAcha Valls, DanielHidalgo Prieto, Manuel2024-02-022024-02-022015-07Effect of single-layer centrifugation or washing on frozen-thawed donkey semen quality: Do they have the same effect regardless of the quality of the sample? Theriogenology. 2015 Jul 15;84(2):294-3000093-691X10.1016/j.theriogenology.2015.03.021https://hdl.handle.net/20.500.14352/98365The aims of this study were to determine the sperm quality of frozen-thawed donkey sperm samples after single-layer centrifugation (SLC) using Androcoll-E in comparison to sperm washing or no centrifugation and to determine if the effect on the sperm quality after SLC or sperm washing depends on the quality of the sample. Frozen-thawed sperm samples from Andalusian donkeys were divided into three aliquots, and they were processed using three different techniques after thawing: uncentrifuged diluted control (UDC), sperm washing (SW), and SLC. Afterward, sperm quality index was estimated by integrating all parameters (total and progressive sperm motility, membrane integrity, and DNA fragmentation) in a single value. The relationship between the sperm quality of thawed UDC samples and the effect on sperm parameters in SW and SLC-selected samples was assessed. Sperm quality index was significantly higher (P < 0.001) in SLC (0.8 ± 0.0) samples than that in UDC (0.6 ± 0.0) and SW (0.6 ± 0.0) samples, regardless of the sperm quality index after thawing of the sperm sample. In conclusion, SLC of frozen-thawed donkey spermatozoa using Androcoll-E-Small can be a suitable procedure for selecting frozen-thawed donkey sperm with better quality, in particular in those samples where an improvement in motility is needed.engAttribution-NonCommercial-NoDerivatives 4.0 Internationaljournal articlehttps://www.sciencedirect.com/science/article/pii/S0093691X15001491?via%3Dihub25917884https://www.sciencedirect.com/journal/theriogenologyrestricted accessDonkeyCryopreservationSingle-layer centrifugationSperm washingSperm qualityVeterinaria24 Ciencias de la Vida