Fernández Bautista, TamaraGómez Gómez, BeatrizGracia Lor, EmmaPérez Corona, María TeresaMadrid Albarrán, María Yolanda2025-11-122025-11-122024-11Fernández-Bautista, Tamara, et al. «Investigating the Presence of Selenoneine, Ergothioneine, and Selenium-Containing Biomolecules in Fish and Fish-Derived Commercial Products». Journal of Agricultural and Food Chemistry, vol. 72, n.o 47, noviembre de 2024, pp. 26155-64. DOI.org (Crossref), https://doi.org/10.1021/acs.jafc.4c06111.10.1021/acs.jafc.4c06111https://hdl.handle.net/20.500.14352/126003Two potent antioxidants, selenoneine and ergothioneine, have been determined simultaneously for the first time in the muscle of highly consumed fish and in fish-derived products. The extraction methods were optimized, and their subsequent analysis was performed by HPLC-ESI-MS/MS. Best extraction efficiency (106 ± 3 and 104 ± 5%) was attained by performing a simple aqueous extraction containing 100 mmol L−1 ammonium acetate or ammonium formate as extractants. Selenoneine and ergothioneine were simultaneously detected in tuna (0.306 ± 0.006 and 3.5 ± 0.2 μg g−1) and swordfish (0.14 ± 0.02 and 1.1 ± 0.4 μg g−1) muscle tissues, while in fish roe and in farmed salmon, only ergothioneine was detected (with contents ranging 0.03−3.3 μg g−1). Moreover, the presence of their respective methylated forms was also considered. Additionally, Se-containing biomolecule profiles from water-soluble proteins were obtained by SEC-ICP-MS analysis. Results revealed similar profiles between fish and fish roe, whereas for ultraprocessed fish products, Se seems to be mostly associated with low-molecular-weight biomolecules. The results included in this work may contribute to a better understanding of the relations between selenoneine and ergothioneine in fishery products as well as their fate and potential modifications of Se-containing biomolecule profiles during fish processing.engjournal articlehttps://doi.org/10.1021/acs.jafc.4c06111https://pubs.acs.org/doi/10.1021/acs.jafc.4c06111restricted access543SelenoneineErgothioneineSelenium biomoleculesFish-derived productsHPLC-ESI-MS/MSHPLC-ICP-MSCiencias23 Química